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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Bartonella tribocorum Bifunctional protein FolD (folD) CSB-YP431883BOS
CSB-EP431883BOS
CSB-BP431883BOS
CSB-MP431883BOS
CSB-EP431883BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum ATP synthase subunit b 2 (atpF2), partial CSB-YP431884BOS
CSB-EP431884BOS
CSB-BP431884BOS
CSB-MP431884BOS
CSB-EP431884BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP431885BPB
CSB-EP431885BPB
CSB-BP431885BPB
CSB-MP431885BPB
CSB-EP431885BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP431886BOS
CSB-EP431886BOS
CSB-BP431886BOS
CSB-MP431886BOS
CSB-EP431886BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP431887BPB
CSB-EP431887BPB
CSB-BP431887BPB
CSB-MP431887BPB
CSB-EP431887BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum Phosphomethylpyrimidine synthase (thiC), partial CSB-YP431888BOS
CSB-EP431888BOS
CSB-BP431888BOS
CSB-MP431888BOS
CSB-EP431888BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP431889BOS
CSB-EP431889BOS
CSB-BP431889BOS
CSB-MP431889BOS
CSB-EP431889BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum Enolase (eno) CSB-YP431890BOS
CSB-EP431890BOS
CSB-BP431890BOS
CSB-MP431890BOS
CSB-EP431890BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Chorismate synthase (aroC) CSB-YP431891BPB
CSB-EP431891BPB
CSB-BP431891BPB
CSB-MP431891BPB
CSB-EP431891BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP431892BPB
CSB-EP431892BPB
CSB-BP431892BPB
CSB-MP431892BPB
CSB-EP431892BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP431893BOS
CSB-EP431893BOS
CSB-BP431893BOS
CSB-MP431893BOS
CSB-EP431893BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum Proline--tRNA ligase (proS) CSB-YP431894BOS
CSB-EP431894BOS
CSB-BP431894BOS
CSB-MP431894BOS
CSB-EP431894BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum Putative membrane protein insertion efficiency factor (BT_1305) CSB-YP431895BOS
CSB-EP431895BOS
CSB-BP431895BOS
CSB-MP431895BOS
CSB-EP431895BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum Bifunctional protein GlmU (glmU) CSB-YP431896BOS
CSB-EP431896BOS
CSB-BP431896BOS
CSB-MP431896BOS
CSB-EP431896BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum Alanine--tRNA ligase (alaS), partial CSB-YP431897BOS
CSB-EP431897BOS
CSB-BP431897BOS
CSB-MP431897BOS
CSB-EP431897BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum 30S ribosomal protein S5 (rpsE) CSB-YP431898BOS
CSB-EP431898BOS
CSB-BP431898BOS
CSB-MP431898BOS
CSB-EP431898BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum 50S ribosomal protein L29 (rpmC) CSB-YP431899BOS
CSB-EP431899BOS
CSB-BP431899BOS
CSB-MP431899BOS
CSB-EP431899BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum 30S ribosomal protein S10 (rpsJ) CSB-YP431900BOS
CSB-EP431900BOS
CSB-BP431900BOS
CSB-MP431900BOS
CSB-EP431900BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum D-alanine--D-alanine ligase (ddl) CSB-YP431901BOS
CSB-EP431901BOS
CSB-BP431901BOS
CSB-MP431901BOS
CSB-EP431901BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP431902BOS
CSB-EP431902BOS
CSB-BP431902BOS
CSB-MP431902BOS
CSB-EP431902BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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