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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Gluconacetobacter diazotrophicus (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP431843GEN
CSB-EP431843GEN
CSB-BP431843GEN
CSB-MP431843GEN
CSB-EP431843GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus Cell division topological specificity factor (minE) CSB-YP431844GEN
CSB-EP431844GEN
CSB-BP431844GEN
CSB-MP431844GEN
CSB-EP431844GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus Biotin synthase (bioB) CSB-YP431845GEN
CSB-EP431845GEN
CSB-BP431845GEN
CSB-MP431845GEN
CSB-EP431845GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus NADH-quinone oxidoreductase subunit N (nuoN), partial CSB-YP431846GEN
CSB-EP431846GEN
CSB-BP431846GEN
CSB-MP431846GEN
CSB-EP431846GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP431847GEN
CSB-EP431847GEN
CSB-BP431847GEN
CSB-MP431847GEN
CSB-EP431847GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Protein smg homolog (smg) CSB-YP431848BPB
CSB-EP431848BPB
CSB-BP431848BPB
CSB-MP431848BPB
CSB-EP431848BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP431849BPB
CSB-EP431849BPB
CSB-BP431849BPB
CSB-MP431849BPB
CSB-EP431849BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Gamma-glutamyl phosphate reductase (proA) CSB-YP431850BPB
CSB-EP431850BPB
CSB-BP431850BPB
CSB-MP431850BPB
CSB-EP431850BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP431851BPB
CSB-EP431851BPB
CSB-BP431851BPB
CSB-MP431851BPB
CSB-EP431851BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Orotate phosphoribosyltransferase (pyrE) CSB-YP431852BPB
CSB-EP431852BPB
CSB-BP431852BPB
CSB-MP431852BPB
CSB-EP431852BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Shikimate dehydrogenase (aroE) CSB-YP431853BPB
CSB-EP431853BPB
CSB-BP431853BPB
CSB-MP431853BPB
CSB-EP431853BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Anhydro-N-acetylmuramic acid kinase (anmK) CSB-YP431854BPB
CSB-EP431854BPB
CSB-BP431854BPB
CSB-MP431854BPB
CSB-EP431854BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP431855BPB
CSB-EP431855BPB
CSB-BP431855BPB
CSB-MP431855BPB
CSB-EP431855BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP431856BPB
CSB-EP431856BPB
CSB-BP431856BPB
CSB-MP431856BPB
CSB-EP431856BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Outer-membrane lipoprotein lolB (lolB) CSB-YP431857BPB
CSB-EP431857BPB
CSB-BP431857BPB
CSB-MP431857BPB
CSB-EP431857BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Periplasmic nitrate reductase (napA), partial CSB-YP431858BPB
CSB-EP431858BPB
CSB-BP431858BPB
CSB-MP431858BPB
CSB-EP431858BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP431859BPB
CSB-EP431859BPB
CSB-BP431859BPB
CSB-MP431859BPB
CSB-EP431859BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii 50S ribosomal protein L27 (rpmA) CSB-YP431860BPB
CSB-EP431860BPB
CSB-BP431860BPB
CSB-MP431860BPB
CSB-EP431860BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP431861BPB
CSB-EP431861BPB
CSB-BP431861BPB
CSB-MP431861BPB
CSB-EP431861BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Aspartate--tRNA ligase (aspS), partial CSB-YP431862BPB
CSB-EP431862BPB
CSB-BP431862BPB
CSB-MP431862BPB
CSB-EP431862BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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