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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Enterocytozoon bieneusi Probable DNA repair helicase RAD25 homolog (RAD25), partial CSB-YP431803EMA
CSB-EP431803EMA
CSB-BP431803EMA
CSB-MP431803EMA
CSB-EP431803EMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterocytozoon bieneusi Probable asparagine--tRNA ligase, cytoplasmic (EBI_22533) CSB-YP431804EMA
CSB-EP431804EMA
CSB-BP431804EMA
CSB-MP431804EMA
CSB-EP431804EMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum Alanine racemase (alr) CSB-YP431805SUD
CSB-EP431805SUD
CSB-BP431805SUD
CSB-MP431805SUD
CSB-EP431805SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP431806SUD1
CSB-EP431806SUD1
CSB-BP431806SUD1
CSB-MP431806SUD1
CSB-EP431806SUD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum Na (+)/H (+) antiporter nhaA 2, partial CSB-YP431807SUD
CSB-EP431807SUD
CSB-BP431807SUD
CSB-MP431807SUD
CSB-EP431807SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum NADH-quinone oxidoreductase subunit N 2 (nuoN2), partial CSB-YP431808SUD
CSB-EP431808SUD
CSB-BP431808SUD
CSB-MP431808SUD
CSB-EP431808SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP431809SUD
CSB-EP431809SUD
CSB-BP431809SUD
CSB-MP431809SUD
CSB-EP431809SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum UPF0753 protein sce4789 (sce4789), partial CSB-YP431810SUD
CSB-EP431810SUD
CSB-BP431810SUD
CSB-MP431810SUD
CSB-EP431810SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum 50S ribosomal protein L21 (rplU) CSB-YP431811SUD
CSB-EP431811SUD
CSB-BP431811SUD
CSB-MP431811SUD
CSB-EP431811SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum 50S ribosomal protein L18 (rplR) CSB-YP431812SUD
CSB-EP431812SUD
CSB-BP431812SUD
CSB-MP431812SUD
CSB-EP431812SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP431813SUD
CSB-EP431813SUD
CSB-BP431813SUD
CSB-MP431813SUD
CSB-EP431813SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum CinA-like protein (sce8168) CSB-YP431814SUD
CSB-EP431814SUD
CSB-BP431814SUD
CSB-MP431814SUD
CSB-EP431814SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP431815SUD
CSB-EP431815SUD
CSB-BP431815SUD
CSB-MP431815SUD
CSB-EP431815SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum SsrA-binding protein (smpB) CSB-YP431816SUD
CSB-EP431816SUD
CSB-BP431816SUD
CSB-MP431816SUD
CSB-EP431816SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum Probable bifunctional SAT/APS kinase 2 (sat2/cysC2), partial CSB-YP431817SUD
CSB-EP431817SUD
CSB-BP431817SUD
CSB-MP431817SUD
CSB-EP431817SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum 60 kDa chaperonin 2 (groL2), partial CSB-YP431818SUD
CSB-EP431818SUD
CSB-BP431818SUD
CSB-MP431818SUD
CSB-EP431818SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP431819SUD
CSB-EP431819SUD
CSB-BP431819SUD
CSB-MP431819SUD
CSB-EP431819SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP431820SUD
CSB-EP431820SUD
CSB-BP431820SUD
CSB-MP431820SUD
CSB-EP431820SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum Probable transaldolase (tal) CSB-YP431821SUD
CSB-EP431821SUD
CSB-BP431821SUD
CSB-MP431821SUD
CSB-EP431821SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP431822SUD
CSB-EP431822SUD
CSB-BP431822SUD
CSB-MP431822SUD
CSB-EP431822SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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