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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus weihenstephanensis 60 kDa chaperonin (groL), partial CSB-YP432423BON
CSB-EP432423BON
CSB-BP432423BON
CSB-MP432423BON
CSB-EP432423BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Deoxyribose-phosphate aldolase (deoC) CSB-YP432424BON
CSB-EP432424BON
CSB-BP432424BON
CSB-MP432424BON
CSB-EP432424BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP432425BON
CSB-EP432425BON
CSB-BP432425BON
CSB-MP432425BON
CSB-EP432425BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Methylthioribose-1-phosphate isomerase 1 (mtnA1) CSB-YP432426BON
CSB-EP432426BON
CSB-BP432426BON
CSB-MP432426BON
CSB-EP432426BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB, partial CSB-YP432427BON
CSB-EP432427BON
CSB-BP432427BON
CSB-MP432427BON
CSB-EP432427BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis ATP synthase epsilon chain (atpC) CSB-YP432428BON
CSB-EP432428BON
CSB-BP432428BON
CSB-MP432428BON
CSB-EP432428BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UPF0374 protein BcerKBAB4_0443 (BcerKBAB4_0443) CSB-YP432429BON
CSB-EP432429BON
CSB-BP432429BON
CSB-MP432429BON
CSB-EP432429BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO CSB-YP432430BON
CSB-EP432430BON
CSB-BP432430BON
CSB-MP432430BON
CSB-EP432430BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP432431BON
CSB-EP432431BON
CSB-BP432431BON
CSB-MP432431BON
CSB-EP432431BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 50S ribosomal protein L9 (rplI) CSB-YP432432BON
CSB-EP432432BON
CSB-BP432432BON
CSB-MP432432BON
CSB-EP432432BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis tRNA modification GTPase MnmE (mnmE) CSB-YP432433BON
CSB-EP432433BON
CSB-BP432433BON
CSB-MP432433BON
CSB-EP432433BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Cell division protein SepF (sepF) CSB-YP432434BON
CSB-EP432434BON
CSB-BP432434BON
CSB-MP432434BON
CSB-EP432434BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UPF0348 protein BcerKBAB4_3752 (BcerKBAB4_3752) CSB-YP432435BON
CSB-EP432435BON
CSB-BP432435BON
CSB-MP432435BON
CSB-EP432435BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP432436MNY
CSB-EP432436MNY
CSB-BP432436MNY
CSB-MP432436MNY
CSB-EP432436MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP432437MNY
CSB-EP432437MNY
CSB-BP432437MNY
CSB-MP432437MNY
CSB-EP432437MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens Putative arginyl-tRNA--protein transferase (ate) CSB-YP432438MNY
CSB-EP432438MNY
CSB-BP432438MNY
CSB-MP432438MNY
CSB-EP432438MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens Orotate phosphoribosyltransferase (pyrE) CSB-YP432439MNY
CSB-EP432439MNY
CSB-BP432439MNY
CSB-MP432439MNY
CSB-EP432439MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP432440MNY
CSB-EP432440MNY
CSB-BP432440MNY
CSB-MP432440MNY
CSB-EP432440MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens ATP-dependent protease subunit HslV (hslV) CSB-YP432441MNY
CSB-EP432441MNY
CSB-BP432441MNY
CSB-MP432441MNY
CSB-EP432441MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP432442MNY
CSB-EP432442MNY
CSB-BP432442MNY
CSB-MP432442MNY
CSB-EP432442MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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