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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Chloroflexus aurantiacus Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP433363DSX
CSB-EP433363DSX
CSB-BP433363DSX
CSB-MP433363DSX
CSB-EP433363DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus Formate--tetrahydrofolate ligase (fhs), partial CSB-YP433364DSX
CSB-EP433364DSX
CSB-BP433364DSX
CSB-MP433364DSX
CSB-EP433364DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus SsrA-binding protein (smpB) CSB-YP433365DSX
CSB-EP433365DSX
CSB-BP433365DSX
CSB-MP433365DSX
CSB-EP433365DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus UPF0102 protein Caur_2698 (Caur_2698) CSB-YP433366DSX
CSB-EP433366DSX
CSB-BP433366DSX
CSB-MP433366DSX
CSB-EP433366DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP433367DSX
CSB-EP433367DSX
CSB-BP433367DSX
CSB-MP433367DSX
CSB-EP433367DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus tRNA modification GTPase MnmE (mnmE) CSB-YP433368DSX
CSB-EP433368DSX
CSB-BP433368DSX
CSB-MP433368DSX
CSB-EP433368DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum Cysteine--tRNA ligase (cysS) CSB-YP433369RIH
CSB-EP433369RIH
CSB-BP433369RIH
CSB-MP433369RIH
CSB-EP433369RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum Phosphoglucosamine mutase (glmM) CSB-YP433370RIH
CSB-EP433370RIH
CSB-BP433370RIH
CSB-MP433370RIH
CSB-EP433370RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum 60 kDa chaperonin 2 (groL2), partial CSB-YP433371RIH
CSB-EP433371RIH
CSB-BP433371RIH
CSB-MP433371RIH
CSB-EP433371RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum Putative endo-alpha-N-acetylgalactosaminidase (RSal33209_1326, RSal33209_1327, RSal33209_1328, RSal33209_1329), partial CSB-YP433372RIH
CSB-EP433372RIH
CSB-BP433372RIH
CSB-MP433372RIH
CSB-EP433372RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum 50S ribosomal protein L19 (rplS) CSB-YP433373RIH
CSB-EP433373RIH
CSB-BP433373RIH
CSB-MP433373RIH
CSB-EP433373RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum Peptide chain release factor 2 (prfB) CSB-YP433374RIH
CSB-EP433374RIH
CSB-BP433374RIH
CSB-MP433374RIH
CSB-EP433374RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum Imidazole glycerol phosphate synthase subunit HisH (hisH) CSB-YP433375RIH
CSB-EP433375RIH
CSB-BP433375RIH
CSB-MP433375RIH
CSB-EP433375RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP433376RIH
CSB-EP433376RIH
CSB-BP433376RIH
CSB-MP433376RIH
CSB-EP433376RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP433377RIH
CSB-EP433377RIH
CSB-BP433377RIH
CSB-MP433377RIH
CSB-EP433377RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum Proteasome-associated ATPase (arc), partial CSB-YP433378RIH
CSB-EP433378RIH
CSB-BP433378RIH
CSB-MP433378RIH
CSB-EP433378RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum Undecaprenyl-diphosphatase (uppP), partial CSB-YP433379RIH
CSB-EP433379RIH
CSB-BP433379RIH
CSB-MP433379RIH
CSB-EP433379RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum Deoxycytidine triphosphate deaminase (dcd) CSB-YP433380RIH
CSB-EP433380RIH
CSB-BP433380RIH
CSB-MP433380RIH
CSB-EP433380RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum 30S ribosomal protein S14 (rpsN) CSB-YP433381RIH
CSB-EP433381RIH
CSB-BP433381RIH
CSB-MP433381RIH
CSB-EP433381RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum Trigger factor (tig) CSB-YP433382RIH
CSB-EP433382RIH
CSB-BP433382RIH
CSB-MP433382RIH
CSB-EP433382RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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