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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Yersinia pestis bv. Antiqua N-succinylarginine dihydrolase (astB) CSB-YP432323YAM
CSB-EP432323YAM
CSB-BP432323YAM
CSB-MP432323YAM
CSB-EP432323YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua UPF0283 membrane protein YpAngola_A2532 (YpAngola_A2532), partial CSB-YP432324YAM1
CSB-EP432324YAM1
CSB-BP432324YAM1
CSB-MP432324YAM1
CSB-EP432324YAM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua HTH-type transcriptional repressor PurR (purR) CSB-YP432325YAM
CSB-EP432325YAM
CSB-BP432325YAM
CSB-MP432325YAM
CSB-EP432325YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Cell division protein ZipA homolog (zipA), partial CSB-YP432326YAM
CSB-EP432326YAM
CSB-BP432326YAM
CSB-MP432326YAM
CSB-EP432326YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP432327YAM
CSB-EP432327YAM
CSB-BP432327YAM
CSB-MP432327YAM
CSB-EP432327YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Dihydrodipicolinate reductase (dapB) CSB-YP432328YAM
CSB-EP432328YAM
CSB-BP432328YAM
CSB-MP432328YAM
CSB-EP432328YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 30S ribosomal protein S20 (rpsT) CSB-YP432329YAM
CSB-EP432329YAM
CSB-BP432329YAM
CSB-MP432329YAM
CSB-EP432329YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Non-canonical purine NTP phosphatase (YpAngola_A0816) CSB-YP432330YAM
CSB-EP432330YAM
CSB-BP432330YAM
CSB-MP432330YAM
CSB-EP432330YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Lipoate-protein ligase A (lplA) CSB-YP432331YAM
CSB-EP432331YAM
CSB-BP432331YAM
CSB-MP432331YAM
CSB-EP432331YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP432332YAM
CSB-EP432332YAM
CSB-BP432332YAM
CSB-MP432332YAM
CSB-EP432332YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Ribonuclease H (rnhA) CSB-YP432333YAM
CSB-EP432333YAM
CSB-BP432333YAM
CSB-MP432333YAM
CSB-EP432333YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Secretion monitor (secM) CSB-YP432334YAM
CSB-EP432334YAM
CSB-BP432334YAM
CSB-MP432334YAM
CSB-EP432334YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 5'/3'-nucleotidase SurE (surE) CSB-YP432335YAM
CSB-EP432335YAM
CSB-BP432335YAM
CSB-MP432335YAM
CSB-EP432335YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP432336YAM
CSB-EP432336YAM
CSB-BP432336YAM
CSB-MP432336YAM
CSB-EP432336YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP432337YAM
CSB-EP432337YAM
CSB-BP432337YAM
CSB-MP432337YAM
CSB-EP432337YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP432338YAM
CSB-EP432338YAM
CSB-BP432338YAM
CSB-MP432338YAM
CSB-EP432338YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua UPF0289 protein YpAngola_A1046 (YpAngola_A1046) CSB-YP432339YAM
CSB-EP432339YAM
CSB-BP432339YAM
CSB-MP432339YAM
CSB-EP432339YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua DnaA initiator-associating protein diaA (diaA) CSB-YP432340YAM
CSB-EP432340YAM
CSB-BP432340YAM
CSB-MP432340YAM
CSB-EP432340YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua p-hydroxybenzoic acid efflux pump subunit AaeB (aaeB), partial CSB-YP432341YAM
CSB-EP432341YAM
CSB-BP432341YAM
CSB-MP432341YAM
CSB-EP432341YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Protein RnfH (rnfH) CSB-YP432342YAM
CSB-EP432342YAM
CSB-BP432342YAM
CSB-MP432342YAM
CSB-EP432342YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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