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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bombyx mori Facilitated trehalose transporter Tret1 (Tret1), partial CSB-YP434283BTT
CSB-EP434283BTT
CSB-BP434283BTT
CSB-MP434283BTT
CSB-EP434283BTT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosopumilus maritimus Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP434284NFB
CSB-EP434284NFB
CSB-BP434284NFB
CSB-MP434284NFB
CSB-EP434284NFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosopumilus maritimus Bifunctional protein FolD (folD) CSB-YP434285NFB
CSB-EP434285NFB
CSB-BP434285NFB
CSB-MP434285NFB
CSB-EP434285NFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosopumilus maritimus Alanine--tRNA ligase (alaS), partial CSB-YP434286NFB
CSB-EP434286NFB
CSB-BP434286NFB
CSB-MP434286NFB
CSB-EP434286NFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosopumilus maritimus 50S ribosomal protein L22P (rpl22p) CSB-YP434287NFB
CSB-EP434287NFB
CSB-BP434287NFB
CSB-MP434287NFB
CSB-EP434287NFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosopumilus maritimus Elongation factor 1-beta (ef1b) CSB-YP434288NFB
CSB-EP434288NFB
CSB-BP434288NFB
CSB-MP434288NFB
CSB-EP434288NFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Probable molybdenum cofactor biosynthesis protein A (moaA) CSB-YP434289MNQ
CSB-EP434289MNQ
CSB-BP434289MNQ
CSB-MP434289MNQ
CSB-EP434289MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase (flpA) CSB-YP434290MNQ
CSB-EP434290MNQ
CSB-BP434290MNQ
CSB-MP434290MNQ
CSB-EP434290MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein (MmarC6_0494), partial CSB-YP434291MNQ
CSB-EP434291MNQ
CSB-BP434291MNQ
CSB-MP434291MNQ
CSB-EP434291MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 2-phospho-L-lactate transferase (cofD) CSB-YP434292MNQ
CSB-EP434292MNQ
CSB-BP434292MNQ
CSB-MP434292MNQ
CSB-EP434292MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Proteasome subunit beta (psmB) CSB-YP434293MNQ
CSB-EP434293MNQ
CSB-BP434293MNQ
CSB-MP434293MNQ
CSB-EP434293MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Ketol-acid reductoisomerase (ilvC) CSB-YP434294MNQ
CSB-EP434294MNQ
CSB-BP434294MNQ
CSB-MP434294MNQ
CSB-EP434294MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L15e (rpl15e) CSB-YP434295MNQ
CSB-EP434295MNQ
CSB-BP434295MNQ
CSB-MP434295MNQ
CSB-EP434295MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 7-cyano-7-deazaguanine synthase (queC) CSB-YP434296MNQ
CSB-EP434296MNQ
CSB-BP434296MNQ
CSB-MP434296MNQ
CSB-EP434296MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Hypoxanthine/guanine phosphoribosyltransferase (hpt) CSB-YP434297MNQ
CSB-EP434297MNQ
CSB-BP434297MNQ
CSB-MP434297MNQ
CSB-EP434297MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP434298MNQ
CSB-EP434298MNQ
CSB-BP434298MNQ
CSB-MP434298MNQ
CSB-EP434298MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Geranylgeranylglyceryl phosphate synthase (MmarC6_0942) CSB-YP434299MNQ
CSB-EP434299MNQ
CSB-BP434299MNQ
CSB-MP434299MNQ
CSB-EP434299MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Digeranylgeranylglyceryl phosphate synthase (MmarC6_0995), partial CSB-YP434300MNQ1
CSB-EP434300MNQ1
CSB-BP434300MNQ1
CSB-MP434300MNQ1
CSB-EP434300MNQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Carbamoyl-phosphate synthase small chain (carA) CSB-YP434301MNQ
CSB-EP434301MNQ
CSB-BP434301MNQ
CSB-MP434301MNQ
CSB-EP434301MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L4P (rpl4p) CSB-YP434302MNQ
CSB-EP434302MNQ
CSB-BP434302MNQ
CSB-MP434302MNQ
CSB-EP434302MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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