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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Yersinia pestis bv. Antiqua Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP434083YAM
CSB-EP434083YAM
CSB-BP434083YAM
CSB-MP434083YAM
CSB-EP434083YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Autoinducer 2 kinase LsrK (lsrK), partial CSB-YP434084YAM
CSB-EP434084YAM
CSB-BP434084YAM
CSB-MP434084YAM
CSB-EP434084YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase (arnT), partial CSB-YP434085YAM
CSB-EP434085YAM
CSB-BP434085YAM
CSB-MP434085YAM
CSB-EP434085YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Vitamin B12 import system permease protein BtuC (btuC), partial CSB-YP434086YAM
CSB-EP434086YAM
CSB-BP434086YAM
CSB-MP434086YAM
CSB-EP434086YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Integration host factor subunit alpha (ihfA) CSB-YP434087YAM
CSB-EP434087YAM
CSB-BP434087YAM
CSB-MP434087YAM
CSB-EP434087YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 50S ribosomal protein L10 (rplJ) CSB-YP434088YAM
CSB-EP434088YAM
CSB-BP434088YAM
CSB-MP434088YAM
CSB-EP434088YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP434089YAM
CSB-EP434089YAM
CSB-BP434089YAM
CSB-MP434089YAM
CSB-EP434089YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua UPF0133 protein YpAngola_A2890 (YpAngola_A2890) CSB-YP434090YAM
CSB-EP434090YAM
CSB-BP434090YAM
CSB-MP434090YAM
CSB-EP434090YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Autoinducer 2-binding protein lsrB (lsrB) CSB-YP434091YAM
CSB-EP434091YAM
CSB-BP434091YAM
CSB-MP434091YAM
CSB-EP434091YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 30S ribosomal protein S16 (rpsP) CSB-YP434092YAM
CSB-EP434092YAM
CSB-BP434092YAM
CSB-MP434092YAM
CSB-EP434092YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Cell division protein FtsB (ftsB), partial CSB-YP434093YAM
CSB-EP434093YAM
CSB-BP434093YAM
CSB-MP434093YAM
CSB-EP434093YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 2-isopropylmalate synthase (leuA), partial CSB-YP434094YAM
CSB-EP434094YAM
CSB-BP434094YAM
CSB-MP434094YAM
CSB-EP434094YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua UPF0231 protein YpAngola_A1027 (YpAngola_A1027) CSB-YP434095YAM
CSB-EP434095YAM
CSB-BP434095YAM
CSB-MP434095YAM
CSB-EP434095YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Aspartate carbamoyltransferase (pyrB) CSB-YP434096YAM
CSB-EP434096YAM
CSB-BP434096YAM
CSB-MP434096YAM
CSB-EP434096YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Protein AaeX (aaeX), partial CSB-YP434097YAM1
CSB-EP434097YAM1
CSB-BP434097YAM1
CSB-MP434097YAM1
CSB-EP434097YAM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua UDP-2,3-diacylglucosamine hydrolase (lpxH) CSB-YP434098YAM
CSB-EP434098YAM
CSB-BP434098YAM
CSB-MP434098YAM
CSB-EP434098YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Ecotin (eco) CSB-YP434099YAM
CSB-EP434099YAM
CSB-BP434099YAM
CSB-MP434099YAM
CSB-EP434099YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP434100YAM
CSB-EP434100YAM
CSB-BP434100YAM
CSB-MP434100YAM
CSB-EP434100YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP434101YAM
CSB-EP434101YAM
CSB-BP434101YAM
CSB-MP434101YAM
CSB-EP434101YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Ribosomal RNA large subunit methyltransferase M (rlmM) CSB-YP434102YAM
CSB-EP434102YAM
CSB-BP434102YAM
CSB-MP434102YAM
CSB-EP434102YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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