Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methanococcus maripaludis tRNA (pseudouridine (54)-N (1))-methyltransferase CSB-YP432543MNQ
CSB-EP432543MNQ
CSB-BP432543MNQ
CSB-MP432543MNQ
CSB-EP432543MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis FO synthase subunit 1 (cofG) CSB-YP432544MNQ
CSB-EP432544MNQ
CSB-BP432544MNQ
CSB-MP432544MNQ
CSB-EP432544MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Flagellar L-ring protein (flgH) CSB-YP432545BPS
CSB-EP432545BPS
CSB-BP432545BPS
CSB-MP432545BPS
CSB-EP432545BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 7-cyano-7-deazaguanine synthase (queC) CSB-YP432546BPS
CSB-EP432546BPS
CSB-BP432546BPS
CSB-MP432546BPS
CSB-EP432546BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Phosphoglucosamine mutase (glmM) CSB-YP432547BPS
CSB-EP432547BPS
CSB-BP432547BPS
CSB-MP432547BPS
CSB-EP432547BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans UvrABC system protein C (uvrC), partial CSB-YP432548BPS
CSB-EP432548BPS
CSB-BP432548BPS
CSB-MP432548BPS
CSB-EP432548BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 50S ribosomal protein L10 (rplJ) CSB-YP432549BPS
CSB-EP432549BPS
CSB-BP432549BPS
CSB-MP432549BPS
CSB-EP432549BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 50S ribosomal protein L3 (rplC) CSB-YP432550BPS
CSB-EP432550BPS
CSB-BP432550BPS
CSB-MP432550BPS
CSB-EP432550BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 50S ribosomal protein L29 (rpmC) CSB-YP432551BPS
CSB-EP432551BPS
CSB-BP432551BPS
CSB-MP432551BPS
CSB-EP432551BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 30S ribosomal protein S8 (rpsH) CSB-YP432552BPS
CSB-EP432552BPS
CSB-BP432552BPS
CSB-MP432552BPS
CSB-EP432552BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP432553BPS
CSB-EP432553BPS
CSB-BP432553BPS
CSB-MP432553BPS
CSB-EP432553BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans D-amino acid dehydrogenase small subunit (dadA) CSB-YP432554BPS
CSB-EP432554BPS
CSB-BP432554BPS
CSB-MP432554BPS
CSB-EP432554BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP432555BPS
CSB-EP432555BPS
CSB-BP432555BPS
CSB-MP432555BPS
CSB-EP432555BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Type III pantothenate kinase (coaX) CSB-YP432556BPS
CSB-EP432556BPS
CSB-BP432556BPS
CSB-MP432556BPS
CSB-EP432556BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Cell division topological specificity factor (minE) CSB-YP432557BPS
CSB-EP432557BPS
CSB-BP432557BPS
CSB-MP432557BPS
CSB-EP432557BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans HPr kinase/phosphorylase (hprK) CSB-YP432558BPS
CSB-EP432558BPS
CSB-BP432558BPS
CSB-MP432558BPS
CSB-EP432558BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Urease subunit beta (ureB) CSB-YP432559BPS
CSB-EP432559BPS
CSB-BP432559BPS
CSB-MP432559BPS
CSB-EP432559BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP432560BPS
CSB-EP432560BPS
CSB-BP432560BPS
CSB-MP432560BPS
CSB-EP432560BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Protein TolB (tolB) CSB-YP432561BPS
CSB-EP432561BPS
CSB-BP432561BPS
CSB-MP432561BPS
CSB-EP432561BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP432562BPS
CSB-EP432562BPS
CSB-BP432562BPS
CSB-MP432562BPS
CSB-EP432562BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>