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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Human POU domain, class 3, transcription factor 4 (POU3F4) CSB-YP018399HU
CSB-EP018399HU
CSB-BP018399HU
CSB-MP018399HU
CSB-EP018399HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Protein farnesyltransferase subunit beta (FNTB) CSB-YP008780BO
CSB-EP008780BO
CSB-BP008780BO
CSB-MP008780BO
CSB-EP008780BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human T-complex protein 1 subunit gamma (CCT3) CSB-YP004857HU
CSB-EP004857HU
CSB-BP004857HU
CSB-MP004857HU
CSB-EP004857HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Alpha-aminoadipic semialdehyde dehydrogenase (ALDH7A1) CSB-YP001579HU
CSB-EP001579HU
CSB-BP001579HU
CSB-MP001579HU
CSB-EP001579HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ubiquitin-conjugating enzyme E2 R1 (CDC34) CSB-YP005004HU
CSB-EP005004HU
CSB-BP005004HU
CSB-MP005004HU
CSB-EP005004HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Inositol polyphosphate 1-phosphatase (Inpp1) CSB-YP011729MO
CSB-EP011729MO
CSB-BP011729MO
CSB-MP011729MO
CSB-EP011729MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Histone H3-like centromeric protein A (CENPA) CSB-YP005205HU
CSB-EP005205HU
CSB-BP005205HU
CSB-MP005205HU
CSB-EP005205HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Neuronal acetylcholine receptor subunit alpha-6 (CHRNA6), partial CSB-YP005392CH
CSB-EP005392CH
CSB-BP005392CH
CSB-MP005392CH
CSB-EP005392CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Probable histidine--tRNA ligase, mitochondrial (HARS2) CSB-YP010138HU
CSB-BP010138HU
CSB-MP010138HU
CSB-EP010138HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Somatostatin receptor type 4 (Sstr4), partial CSB-YP022727MO1
CSB-EP022727MO1
CSB-BP022727MO1
CSB-MP022727MO1
CSB-EP022727MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dog Somatostatin (SST) CSB-YP022723DO
CSB-EP022723DO
CSB-BP022723DO
CSB-MP022723DO
CSB-EP022723DO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Proteasome subunit beta type-2 (PSMB2) CSB-YP018879HU
CSB-BP018879HU
CSB-MP018879HU
CSB-EP018879HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Thrombospondin-4 (Thbs4), partial CSB-YP023490RA
CSB-EP023490RA
CSB-BP023490RA
CSB-MP023490RA
CSB-EP023490RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Acyl-coenzyme A thioesterase 2, mitochondrial (ACOT2), partial CSB-YP001166HU
CSB-EP001166HU
CSB-BP001166HU
CSB-MP001166HU
CSB-EP001166HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Placenta growth factor (Pgf) CSB-YP017854MO
CSB-EP017854MO
CSB-BP017854MO
CSB-MP017854MO
CSB-EP017854MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Regulator of G-protein signaling 6 (Rgs6) CSB-YP019658RA
CSB-EP019658RA
CSB-BP019658RA
CSB-MP019658RA
CSB-EP019658RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Regulator of G-protein signaling 10 (Rgs10) CSB-YP019642RA
CSB-EP019642RA
CSB-BP019642RA
CSB-MP019642RA
CSB-EP019642RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guinea pig Estrogen sulfotransferase (SULT1E1) CSB-YP022941GU
CSB-EP022941GU
CSB-BP022941GU
CSB-MP022941GU
CSB-EP022941GU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Sperm mitochondrial-associated cysteine-rich protein (SMCP) CSB-YP021820HU
CSB-BP021820HU
CSB-MP021820HU
CSB-EP021820HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Acidic leucine-rich nuclear phosphoprotein 32 family member A (Anp32a) CSB-YP001822RA
CSB-EP001822RA
CSB-BP001822RA
CSB-MP001822RA
CSB-EP001822RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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