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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Prochlorococcus marinus Cell division topological specificity factor (minE) CSB-YP432623PZC
CSB-EP432623PZC
CSB-BP432623PZC
CSB-MP432623PZC
CSB-EP432623PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Elongation factor 4 (lepA), partial CSB-YP432624PZC
CSB-EP432624PZC
CSB-BP432624PZC
CSB-MP432624PZC
CSB-EP432624PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Light-independent protochlorophyllide reductase subunit B (chlB), partial CSB-YP432625PZC
CSB-EP432625PZC
CSB-BP432625PZC
CSB-MP432625PZC
CSB-EP432625PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Elongation factor P (efp) CSB-YP432626PYI
CSB-EP432626PYI
CSB-BP432626PYI
CSB-MP432626PYI
CSB-EP432626PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Serine--tRNA ligase (serS) CSB-YP432627PYI
CSB-EP432627PYI
CSB-BP432627PYI
CSB-MP432627PYI
CSB-EP432627PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP432628PYI
CSB-EP432628PYI
CSB-BP432628PYI
CSB-MP432628PYI
CSB-EP432628PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis UPF0042 nucleotide-binding protein Pmob_0154 (Pmob_0154) CSB-YP432629PYI
CSB-EP432629PYI
CSB-BP432629PYI
CSB-MP432629PYI
CSB-EP432629PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP432630PYI
CSB-EP432630PYI
CSB-BP432630PYI
CSB-MP432630PYI
CSB-EP432630PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis ATP synthase subunit c (atpE), partial CSB-YP432631PYI1
CSB-EP432631PYI1
CSB-BP432631PYI1
CSB-MP432631PYI1
CSB-EP432631PYI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis 50S ribosomal protein L15 (rplO) CSB-YP432632PYI
CSB-EP432632PYI
CSB-BP432632PYI
CSB-MP432632PYI
CSB-EP432632PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Protein translocase subunit SecF (secF), partial CSB-YP432633PYI1
CSB-EP432633PYI1
CSB-BP432633PYI1
CSB-MP432633PYI1
CSB-EP432633PYI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP432634PYI
CSB-EP432634PYI
CSB-BP432634PYI
CSB-MP432634PYI
CSB-EP432634PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis 30S ribosomal protein S2 (rpsB) CSB-YP432635PYI
CSB-EP432635PYI
CSB-BP432635PYI
CSB-MP432635PYI
CSB-EP432635PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis 50S ribosomal protein L9 (rplI) CSB-YP432636PYI
CSB-EP432636PYI
CSB-BP432636PYI
CSB-MP432636PYI
CSB-EP432636PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis 30S ribosomal protein S19 (rpsS) CSB-YP432637PYI
CSB-EP432637PYI
CSB-BP432637PYI
CSB-MP432637PYI
CSB-EP432637PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis 30S ribosomal protein S12 (rpsL) CSB-YP432638PYI
CSB-EP432638PYI
CSB-BP432638PYI
CSB-MP432638PYI
CSB-EP432638PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Peptide chain release factor 2 (prfB) CSB-YP432639PYI
CSB-EP432639PYI
CSB-BP432639PYI
CSB-MP432639PYI
CSB-EP432639PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP432640PYI
CSB-EP432640PYI
CSB-BP432640PYI
CSB-MP432640PYI
CSB-EP432640PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Anthranilate phosphoribosyltransferase (trpD) CSB-YP432641PYI
CSB-EP432641PYI
CSB-BP432641PYI
CSB-MP432641PYI
CSB-EP432641PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis S-adenosylmethionine synthase (metK) CSB-YP432642PYI
CSB-EP432642PYI
CSB-BP432642PYI
CSB-MP432642PYI
CSB-EP432642PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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