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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Coxiella burnetii NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP434023CXH
CSB-EP434023CXH
CSB-BP434023CXH
CSB-MP434023CXH
CSB-EP434023CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 50S ribosomal protein L7/L12 (rplL) CSB-YP434024CXH
CSB-EP434024CXH
CSB-BP434024CXH
CSB-MP434024CXH
CSB-EP434024CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 30S ribosomal protein S10 (rpsJ) CSB-YP434025CXH
CSB-EP434025CXH
CSB-BP434025CXH
CSB-MP434025CXH
CSB-EP434025CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 50S ribosomal protein L23 (rplW) CSB-YP434026CXH
CSB-EP434026CXH
CSB-BP434026CXH
CSB-MP434026CXH
CSB-EP434026CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Erythronate-4-phosphate dehydrogenase (pdxB) CSB-YP434027CXH
CSB-EP434027CXH
CSB-BP434027CXH
CSB-MP434027CXH
CSB-EP434027CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 2-nonaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (coq7) CSB-YP434028CXH
CSB-EP434028CXH
CSB-BP434028CXH
CSB-MP434028CXH
CSB-EP434028CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 50S ribosomal protein L5 (rplE) CSB-YP434029CXH
CSB-EP434029CXH
CSB-BP434029CXH
CSB-MP434029CXH
CSB-EP434029CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 50S ribosomal protein L30 (rpmD) CSB-YP434030CXH
CSB-EP434030CXH
CSB-BP434030CXH
CSB-MP434030CXH
CSB-EP434030CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 30S ribosomal protein S13 (rpsM) CSB-YP434031CXH
CSB-EP434031CXH
CSB-BP434031CXH
CSB-MP434031CXH
CSB-EP434031CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Putative reductase COXBURSA331_A0369 (COXBURSA331_A0369) CSB-YP434032CXH
CSB-EP434032CXH
CSB-BP434032CXH
CSB-MP434032CXH
CSB-EP434032CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP434033CXH
CSB-EP434033CXH
CSB-BP434033CXH
CSB-MP434033CXH
CSB-EP434033CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Ribonuclease H (rnhA) CSB-YP434034CXH
CSB-EP434034CXH
CSB-BP434034CXH
CSB-MP434034CXH
CSB-EP434034CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 3-dehydroquinate synthase (aroB) CSB-YP434035CXH
CSB-EP434035CXH
CSB-BP434035CXH
CSB-MP434035CXH
CSB-EP434035CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii tRNA modification GTPase MnmE (mnmE) CSB-YP434036CXH
CSB-EP434036CXH
CSB-BP434036CXH
CSB-MP434036CXH
CSB-EP434036CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP434037CXH
CSB-EP434037CXH
CSB-BP434037CXH
CSB-MP434037CXH
CSB-EP434037CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii tRNA (guanine-N (1)-)-methyltransferase CSB-YP434038CXH
CSB-EP434038CXH
CSB-BP434038CXH
CSB-MP434038CXH
CSB-EP434038CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Putative Holliday junction resolvase (COXBURSA331_A2220) CSB-YP434039CXH
CSB-EP434039CXH
CSB-BP434039CXH
CSB-MP434039CXH
CSB-EP434039CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP434040CXH
CSB-EP434040CXH
CSB-BP434040CXH
CSB-MP434040CXH
CSB-EP434040CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP434041CXH
CSB-EP434041CXH
CSB-BP434041CXH
CSB-MP434041CXH
CSB-EP434041CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP434042CXH
CSB-EP434042CXH
CSB-BP434042CXH
CSB-MP434042CXH
CSB-EP434042CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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