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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella arizonae Carboxylate-amine ligase YbdK (ybdK) CSB-YP433023STE
CSB-EP433023STE
CSB-BP433023STE
CSB-MP433023STE
CSB-EP433023STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae C4-dicarboxylate transport protein (dctA), partial CSB-YP433024STE
CSB-EP433024STE
CSB-BP433024STE
CSB-MP433024STE
CSB-EP433024STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP433025STE
CSB-EP433025STE
CSB-BP433025STE
CSB-MP433025STE
CSB-EP433025STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae tRNA sulfurtransferase (thiI) CSB-YP433026STE
CSB-EP433026STE
CSB-BP433026STE
CSB-MP433026STE
CSB-EP433026STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP433027STE
CSB-EP433027STE
CSB-BP433027STE
CSB-MP433027STE
CSB-EP433027STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP433028STE
CSB-EP433028STE
CSB-BP433028STE
CSB-MP433028STE
CSB-EP433028STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Sulfurtransferase TusD (tusD) CSB-YP433029STE
CSB-EP433029STE
CSB-BP433029STE
CSB-MP433029STE
CSB-EP433029STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae 50S ribosomal protein L4 (rplD) CSB-YP433030STE
CSB-EP433030STE
CSB-BP433030STE
CSB-MP433030STE
CSB-EP433030STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae 30S ribosomal protein S17 (rpsQ) CSB-YP433031STE
CSB-EP433031STE
CSB-BP433031STE
CSB-MP433031STE
CSB-EP433031STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae 50S ribosomal protein L6 (rplF) CSB-YP433032STE
CSB-EP433032STE
CSB-BP433032STE
CSB-MP433032STE
CSB-EP433032STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Ribosomal RNA small subunit methyltransferase B (rsmB) CSB-YP433033STE
CSB-EP433033STE
CSB-BP433033STE
CSB-MP433033STE
CSB-EP433033STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Probable transcriptional regulatory protein YebC (yebC) CSB-YP433034STE
CSB-EP433034STE
CSB-BP433034STE
CSB-MP433034STE
CSB-EP433034STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Ribosomal RNA small subunit methyltransferase F (rsmF) CSB-YP433035STE
CSB-EP433035STE
CSB-BP433035STE
CSB-MP433035STE
CSB-EP433035STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Gamma-glutamyl phosphate reductase (proA) CSB-YP433036STE
CSB-EP433036STE
CSB-BP433036STE
CSB-MP433036STE
CSB-EP433036STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Sulfoxide reductase catalytic subunit YedY (yedY) CSB-YP433037STE
CSB-EP433037STE
CSB-BP433037STE
CSB-MP433037STE
CSB-EP433037STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Transcriptional regulator NanR (nanR) CSB-YP433038STE
CSB-EP433038STE
CSB-BP433038STE
CSB-MP433038STE
CSB-EP433038STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP433039STE
CSB-EP433039STE
CSB-BP433039STE
CSB-MP433039STE
CSB-EP433039STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Cell division topological specificity factor (minE) CSB-YP433040STE
CSB-EP433040STE
CSB-BP433040STE
CSB-MP433040STE
CSB-EP433040STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae D-amino acid dehydrogenase small subunit (dadA) CSB-YP433041STE
CSB-EP433041STE
CSB-BP433041STE
CSB-MP433041STE
CSB-EP433041STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae UPF0263 protein yciU (yciU) CSB-YP433042STE
CSB-EP433042STE
CSB-BP433042STE
CSB-MP433042STE
CSB-EP433042STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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