Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Brucella canis 30S ribosomal protein S19 (rpsS) CSB-YP432943BPH
CSB-EP432943BPH
CSB-BP432943BPH
CSB-MP432943BPH
CSB-EP432943BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis 50S ribosomal protein L23 (rplW) CSB-YP432944BPH
CSB-EP432944BPH
CSB-BP432944BPH
CSB-MP432944BPH
CSB-EP432944BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis 50S ribosomal protein L1 (rplA) CSB-YP432945BPH
CSB-EP432945BPH
CSB-BP432945BPH
CSB-MP432945BPH
CSB-EP432945BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Putative cobalt-precorrin-6A synthase [deacetylating] (cbiD) CSB-YP432946BPH
CSB-EP432946BPH
CSB-BP432946BPH
CSB-MP432946BPH
CSB-EP432946BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis DNA ligase (ligA), partial CSB-YP432947BPH
CSB-EP432947BPH
CSB-BP432947BPH
CSB-MP432947BPH
CSB-EP432947BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP432948BPH
CSB-EP432948BPH
CSB-BP432948BPH
CSB-MP432948BPH
CSB-EP432948BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP432949BPH
CSB-EP432949BPH
CSB-BP432949BPH
CSB-MP432949BPH
CSB-EP432949BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP432950BPH
CSB-EP432950BPH
CSB-BP432950BPH
CSB-MP432950BPH
CSB-EP432950BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Lipoprotein signal peptidase (lspA), partial CSB-YP432951BPH1
CSB-EP432951BPH1
CSB-BP432951BPH1
CSB-MP432951BPH1
CSB-EP432951BPH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis tRNA (Ile)-lysidine synthase CSB-YP432952BPH
CSB-EP432952BPH
CSB-BP432952BPH
CSB-MP432952BPH
CSB-EP432952BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis UPF0335 protein BCAN_A1790 (BCAN_A1790) CSB-YP432953BPH
CSB-EP432953BPH
CSB-BP432953BPH
CSB-MP432953BPH
CSB-EP432953BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Urease accessory protein UreF 1 (ureF1) CSB-YP432954BPH
CSB-EP432954BPH
CSB-BP432954BPH
CSB-MP432954BPH
CSB-EP432954BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis 4-hydroxyproline epimerase (BCAN_A1830) CSB-YP432955BPH
CSB-EP432955BPH
CSB-BP432955BPH
CSB-MP432955BPH
CSB-EP432955BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis 50S ribosomal protein L27 (rpmA) CSB-YP432956BPH
CSB-EP432956BPH
CSB-BP432956BPH
CSB-MP432956BPH
CSB-EP432956BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis ATP synthase subunit b 2 (atpF2), partial CSB-YP432957BPH
CSB-EP432957BPH
CSB-BP432957BPH
CSB-MP432957BPH
CSB-EP432957BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP432958BPH
CSB-EP432958BPH
CSB-BP432958BPH
CSB-MP432958BPH
CSB-EP432958BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis 2-oxoglutarate dehydrogenase E1 component (sucA), partial CSB-YP432959BPH
CSB-EP432959BPH
CSB-BP432959BPH
CSB-MP432959BPH
CSB-EP432959BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis 50S ribosomal protein L9 (rplI) CSB-YP432960BPH
CSB-EP432960BPH
CSB-BP432960BPH
CSB-MP432960BPH
CSB-EP432960BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Cytochrome c oxidase assembly protein CtaG (ctaG), partial CSB-YP432961BPH
CSB-EP432961BPH
CSB-BP432961BPH
CSB-MP432961BPH
CSB-EP432961BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Shikimate kinase (aroK) CSB-YP432962BPH
CSB-EP432962BPH
CSB-BP432962BPH
CSB-MP432962BPH
CSB-EP432962BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>