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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Methylobacterium extorquens Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP433343MNY
CSB-EP433343MNY
CSB-BP433343MNY
CSB-MP433343MNY
CSB-EP433343MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP433344MNY1
CSB-EP433344MNY1
CSB-BP433344MNY1
CSB-MP433344MNY1
CSB-EP433344MNY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens 50S ribosomal protein L9 (rplI) CSB-YP433345MNY
CSB-EP433345MNY
CSB-BP433345MNY
CSB-MP433345MNY
CSB-EP433345MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP433346MNY
CSB-EP433346MNY
CSB-BP433346MNY
CSB-MP433346MNY
CSB-EP433346MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus 4-hydroxy-2-oxovalerate aldolase (Caur_1351) CSB-YP433347DSX
CSB-EP433347DSX
CSB-BP433347DSX
CSB-MP433347DSX
CSB-EP433347DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus Cell division topological specificity factor (minE) CSB-YP433348DSX
CSB-EP433348DSX
CSB-BP433348DSX
CSB-MP433348DSX
CSB-EP433348DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus Anthranilate phosphoribosyltransferase (trpD) CSB-YP433349DSX
CSB-EP433349DSX
CSB-BP433349DSX
CSB-MP433349DSX
CSB-EP433349DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus tRNA (guanine-N (1)-)-methyltransferase CSB-YP433350DSX
CSB-EP433350DSX
CSB-BP433350DSX
CSB-MP433350DSX
CSB-EP433350DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus D-tyrosyl-tRNA (Tyr) deacylase CSB-YP433351DSX
CSB-EP433351DSX
CSB-BP433351DSX
CSB-MP433351DSX
CSB-EP433351DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus Arginine--tRNA ligase (argS), partial CSB-YP433352DSX
CSB-EP433352DSX
CSB-BP433352DSX
CSB-MP433352DSX
CSB-EP433352DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus 30S ribosomal protein S16 (rpsP) CSB-YP433353DSX
CSB-EP433353DSX
CSB-BP433353DSX
CSB-MP433353DSX
CSB-EP433353DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP433354DSX
CSB-EP433354DSX
CSB-BP433354DSX
CSB-MP433354DSX
CSB-EP433354DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus Sulfate adenylyltransferase (sat) CSB-YP433355DSX
CSB-EP433355DSX
CSB-BP433355DSX
CSB-MP433355DSX
CSB-EP433355DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP433356DSX
CSB-EP433356DSX
CSB-BP433356DSX
CSB-MP433356DSX
CSB-EP433356DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP433357DSX
CSB-EP433357DSX
CSB-BP433357DSX
CSB-MP433357DSX
CSB-EP433357DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP433358DSX
CSB-EP433358DSX
CSB-BP433358DSX
CSB-MP433358DSX
CSB-EP433358DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus Ribosome-binding factor A (rbfA) CSB-YP433359DSX
CSB-EP433359DSX
CSB-BP433359DSX
CSB-MP433359DSX
CSB-EP433359DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus 30S ribosomal protein S3 (rpsC) CSB-YP433360DSX
CSB-EP433360DSX
CSB-BP433360DSX
CSB-MP433360DSX
CSB-EP433360DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus 30S ribosomal protein S5 (rpsE) CSB-YP433361DSX
CSB-EP433361DSX
CSB-BP433361DSX
CSB-MP433361DSX
CSB-EP433361DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus Chorismate synthase (aroC) CSB-YP433362DSX
CSB-EP433362DSX
CSB-BP433362DSX
CSB-MP433362DSX
CSB-EP433362DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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