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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Burkholderia multivorans tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP434343BPS
CSB-EP434343BPS
CSB-BP434343BPS
CSB-MP434343BPS
CSB-EP434343BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP434344BPS
CSB-EP434344BPS
CSB-BP434344BPS
CSB-MP434344BPS
CSB-EP434344BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 2-isopropylmalate synthase (leuA), partial CSB-YP434345BPS
CSB-EP434345BPS
CSB-BP434345BPS
CSB-MP434345BPS
CSB-EP434345BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans UPF0061 protein Bmul_1366/BMULJ_01877 (Bmul_1366, BMULJ_01877), partial CSB-YP434346BPS
CSB-EP434346BPS
CSB-BP434346BPS
CSB-MP434346BPS
CSB-EP434346BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans UPF0502 protein Bmul_3231/BMULJ_05293 (Bmul_3231, BMULJ_05293) CSB-YP434347BPS
CSB-EP434347BPS
CSB-BP434347BPS
CSB-MP434347BPS
CSB-EP434347BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Betaine aldehyde dehydrogenase (betB) CSB-YP434348BPS
CSB-EP434348BPS
CSB-BP434348BPS
CSB-MP434348BPS
CSB-EP434348BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans UPF0317 protein Bmul_5125/BMULJ_03391 (Bmul_5125, BMULJ_03391) CSB-YP434349BPS
CSB-EP434349BPS
CSB-BP434349BPS
CSB-MP434349BPS
CSB-EP434349BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus Phosphopantetheine adenylyltransferase (coaD) CSB-YP434350HSK
CSB-EP434350HSK
CSB-BP434350HSK
CSB-MP434350HSK
CSB-EP434350HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus Putative membrane protein insertion efficiency factor (Haur_0765) CSB-YP434351HSK
CSB-EP434351HSK
CSB-BP434351HSK
CSB-MP434351HSK
CSB-EP434351HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP434352HSK
CSB-EP434352HSK
CSB-BP434352HSK
CSB-MP434352HSK
CSB-EP434352HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus Phosphate acyltransferase (plsX) CSB-YP434353HSK
CSB-EP434353HSK
CSB-BP434353HSK
CSB-MP434353HSK
CSB-EP434353HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus Probable transaldolase (tal) CSB-YP434354HSK
CSB-EP434354HSK
CSB-BP434354HSK
CSB-MP434354HSK
CSB-EP434354HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus Urease subunit beta (ureB) CSB-YP434355HSK
CSB-EP434355HSK
CSB-BP434355HSK
CSB-MP434355HSK
CSB-EP434355HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP434356HSK
CSB-EP434356HSK
CSB-BP434356HSK
CSB-MP434356HSK
CSB-EP434356HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP434357HSK
CSB-EP434357HSK
CSB-BP434357HSK
CSB-MP434357HSK
CSB-EP434357HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus Elongation factor P (efp) CSB-YP434358HSK
CSB-EP434358HSK
CSB-BP434358HSK
CSB-MP434358HSK
CSB-EP434358HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus Probable RNA 2'-phosphotransferase (kptA) CSB-YP434359HSK
CSB-EP434359HSK
CSB-BP434359HSK
CSB-MP434359HSK
CSB-EP434359HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus Pantothenate synthetase (panC) CSB-YP434360HSK
CSB-EP434360HSK
CSB-BP434360HSK
CSB-MP434360HSK
CSB-EP434360HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus 50S ribosomal protein L22 (rplV) CSB-YP434361HSK
CSB-EP434361HSK
CSB-BP434361HSK
CSB-MP434361HSK
CSB-EP434361HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus 50S ribosomal protein L6 (rplF) CSB-YP434362HSK
CSB-EP434362HSK
CSB-BP434362HSK
CSB-MP434362HSK
CSB-EP434362HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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