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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella arizonae 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP434743STE
CSB-EP434743STE
CSB-BP434743STE
CSB-MP434743STE
CSB-EP434743STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Integration host factor subunit alpha (ihfA) CSB-YP434744STE
CSB-EP434744STE
CSB-BP434744STE
CSB-MP434744STE
CSB-EP434744STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae 50S ribosomal protein L20 (rplT) CSB-YP434745STE
CSB-EP434745STE
CSB-BP434745STE
CSB-MP434745STE
CSB-EP434745STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Adenylosuccinate synthetase (purA) CSB-YP434746STE
CSB-EP434746STE
CSB-BP434746STE
CSB-MP434746STE
CSB-EP434746STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Fumarate reductase subunit D (frdD), partial CSB-YP434747STE1
CSB-EP434747STE1
CSB-BP434747STE1
CSB-MP434747STE1
CSB-EP434747STE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Alanine--tRNA ligase (alaS), partial CSB-YP434748STE
CSB-EP434748STE
CSB-BP434748STE
CSB-MP434748STE
CSB-EP434748STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP434749STE
CSB-EP434749STE
CSB-BP434749STE
CSB-MP434749STE
CSB-EP434749STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Cation/acetate symporter ActP (actP), partial CSB-YP434750STE
CSB-EP434750STE
CSB-BP434750STE
CSB-MP434750STE
CSB-EP434750STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Ribosome maturation factor RimM (rimM) CSB-YP434751STE
CSB-EP434751STE
CSB-BP434751STE
CSB-MP434751STE
CSB-EP434751STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Membrane-bound lytic murein transglycosylase F (mltF) CSB-YP434752STE
CSB-EP434752STE
CSB-BP434752STE
CSB-MP434752STE
CSB-EP434752STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Curli assembly protein CsgC (SARI_01853) CSB-YP434753STE
CSB-EP434753STE
CSB-BP434753STE
CSB-MP434753STE
CSB-EP434753STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP434754STE
CSB-EP434754STE
CSB-BP434754STE
CSB-MP434754STE
CSB-EP434754STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae HTH-type transcriptional repressor fabR (fabR) CSB-YP434755STE
CSB-EP434755STE
CSB-BP434755STE
CSB-MP434755STE
CSB-EP434755STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Heat shock protein hspQ (hspQ) CSB-YP434756STE
CSB-EP434756STE
CSB-BP434756STE
CSB-MP434756STE
CSB-EP434756STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP434757STE
CSB-EP434757STE
CSB-BP434757STE
CSB-MP434757STE
CSB-EP434757STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Serine--tRNA ligase (serS) CSB-YP434758STE
CSB-EP434758STE
CSB-BP434758STE
CSB-MP434758STE
CSB-EP434758STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Glycerol kinase (glpK), partial CSB-YP434759STE
CSB-EP434759STE
CSB-BP434759STE
CSB-MP434759STE
CSB-EP434759STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP434760STE
CSB-EP434760STE
CSB-BP434760STE
CSB-MP434760STE
CSB-EP434760STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae UPF0145 protein ybjQ (ybjQ) CSB-YP434761STE
CSB-EP434761STE
CSB-BP434761STE
CSB-MP434761STE
CSB-EP434761STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP434762STE
CSB-EP434762STE
CSB-BP434762STE
CSB-MP434762STE
CSB-EP434762STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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