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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus weihenstephanensis 30S ribosomal protein S6 (rpsF) CSB-YP433323BON
CSB-EP433323BON
CSB-BP433323BON
CSB-MP433323BON
CSB-EP433323BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Sec-independent protein translocase protein TatA (tatA), partial CSB-YP433324BON
CSB-EP433324BON
CSB-BP433324BON
CSB-MP433324BON
CSB-EP433324BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UPF0747 protein BcerKBAB4_3746 (BcerKBAB4_3746), partial CSB-YP433325BON
CSB-EP433325BON
CSB-BP433325BON
CSB-MP433325BON
CSB-EP433325BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UPF0637 protein BcerKBAB4_3786 (BcerKBAB4_3786) CSB-YP433326BON
CSB-EP433326BON
CSB-BP433326BON
CSB-MP433326BON
CSB-EP433326BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP433327MNY
CSB-EP433327MNY
CSB-BP433327MNY
CSB-MP433327MNY
CSB-EP433327MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO CSB-YP433328MNY
CSB-EP433328MNY
CSB-BP433328MNY
CSB-MP433328MNY
CSB-EP433328MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens SsrA-binding protein (smpB) CSB-YP433329MNY
CSB-EP433329MNY
CSB-BP433329MNY
CSB-MP433329MNY
CSB-EP433329MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens Ribosome maturation factor RimM (rimM) CSB-YP433330MNY
CSB-EP433330MNY
CSB-BP433330MNY
CSB-MP433330MNY
CSB-EP433330MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens Chaperone protein htpG (htpG), partial CSB-YP433331MNY
CSB-EP433331MNY
CSB-BP433331MNY
CSB-MP433331MNY
CSB-EP433331MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens Urease accessory protein UreD 1 (ureD1) CSB-YP433332MNY
CSB-EP433332MNY
CSB-BP433332MNY
CSB-MP433332MNY
CSB-EP433332MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP433333MNY
CSB-EP433333MNY
CSB-BP433333MNY
CSB-MP433333MNY
CSB-EP433333MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens 50S ribosomal protein L35 (rpmI) CSB-YP433334MNY
CSB-EP433334MNY
CSB-BP433334MNY
CSB-MP433334MNY
CSB-EP433334MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP433335MNY
CSB-EP433335MNY
CSB-BP433335MNY
CSB-MP433335MNY
CSB-EP433335MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens Pyrimidine monooxygenase RutA (rutA) CSB-YP433336MNY
CSB-EP433336MNY
CSB-BP433336MNY
CSB-MP433336MNY
CSB-EP433336MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens 30S ribosomal protein S7 (rpsG) CSB-YP433337MNY
CSB-EP433337MNY
CSB-BP433337MNY
CSB-MP433337MNY
CSB-EP433337MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens 30S ribosomal protein S19 (rpsS) CSB-YP433338MNY
CSB-EP433338MNY
CSB-BP433338MNY
CSB-MP433338MNY
CSB-EP433338MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens 50S ribosomal protein L30 (rpmD) CSB-YP433339MNY
CSB-EP433339MNY
CSB-BP433339MNY
CSB-MP433339MNY
CSB-EP433339MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP433340MNY
CSB-EP433340MNY
CSB-BP433340MNY
CSB-MP433340MNY
CSB-EP433340MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens 50S ribosomal protein L34 (rpmH) CSB-YP433341MNY
CSB-EP433341MNY
CSB-BP433341MNY
CSB-MP433341MNY
CSB-EP433341MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens 50S ribosomal protein L25 (rplY) CSB-YP433342MNY
CSB-EP433342MNY
CSB-BP433342MNY
CSB-MP433342MNY
CSB-EP433342MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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