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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella arizonae UPF0115 protein yfcN (yfcN) CSB-YP434763STE
CSB-EP434763STE
CSB-BP434763STE
CSB-MP434763STE
CSB-EP434763STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Erythronate-4-phosphate dehydrogenase (pdxB) CSB-YP434764STE
CSB-EP434764STE
CSB-BP434764STE
CSB-MP434764STE
CSB-EP434764STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP434765STE
CSB-EP434765STE
CSB-BP434765STE
CSB-MP434765STE
CSB-EP434765STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Deubiquitinase sseL (sseL) CSB-YP434766STE
CSB-EP434766STE
CSB-BP434766STE
CSB-MP434766STE
CSB-EP434766STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Biotin synthase (bioB) CSB-YP434767STE
CSB-EP434767STE
CSB-BP434767STE
CSB-MP434767STE
CSB-EP434767STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Ribonuclease P protein component (rnpA) CSB-YP434768STE
CSB-EP434768STE
CSB-BP434768STE
CSB-MP434768STE
CSB-EP434768STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae UPF0352 protein yejL (yejL) CSB-YP434769STE
CSB-EP434769STE
CSB-BP434769STE
CSB-MP434769STE
CSB-EP434769STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Glucosamine-6-phosphate deaminase (nagB) CSB-YP434770STE
CSB-EP434770STE
CSB-BP434770STE
CSB-MP434770STE
CSB-EP434770STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Ribonuclease PH (rph) CSB-YP434771STE
CSB-EP434771STE
CSB-BP434771STE
CSB-MP434771STE
CSB-EP434771STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae UPF0299 membrane protein yohJ (yohJ), partial CSB-YP434772STE1
CSB-EP434772STE1
CSB-BP434772STE1
CSB-MP434772STE1
CSB-EP434772STE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Multidrug resistance protein MdtC (mdtC), partial CSB-YP434773STE
CSB-EP434773STE
CSB-BP434773STE
CSB-MP434773STE
CSB-EP434773STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae L-lactate dehydrogenase [cytochrome] (lldD) CSB-YP434774STE
CSB-EP434774STE
CSB-BP434774STE
CSB-MP434774STE
CSB-EP434774STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae DNA gyrase inhibitor (sbmC) CSB-YP434775STE
CSB-EP434775STE
CSB-BP434775STE
CSB-MP434775STE
CSB-EP434775STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Cobalt transport protein CbiN (cbiN), partial CSB-YP434776STE1
CSB-EP434776STE1
CSB-BP434776STE1
CSB-MP434776STE1
CSB-EP434776STE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae UPF0133 protein ybaB (ybaB) CSB-YP434777STE
CSB-EP434777STE
CSB-BP434777STE
CSB-MP434777STE
CSB-EP434777STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Rhomboid protease glpG (glpG), partial CSB-YP434778STE1
CSB-EP434778STE1
CSB-BP434778STE1
CSB-MP434778STE1
CSB-EP434778STE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Fe/S biogenesis protein NfuA (nfuA) CSB-YP434779STE
CSB-EP434779STE
CSB-BP434779STE
CSB-MP434779STE
CSB-EP434779STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Flagellar transcriptional regulator FlhD (flhD) CSB-YP434780STE
CSB-EP434780STE
CSB-BP434780STE
CSB-MP434780STE
CSB-EP434780STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Protein TusB (tusB) CSB-YP434781STE
CSB-EP434781STE
CSB-BP434781STE
CSB-MP434781STE
CSB-EP434781STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae 30S ribosomal protein S10 (rpsJ) CSB-YP434782STE
CSB-EP434782STE
CSB-BP434782STE
CSB-MP434782STE
CSB-EP434782STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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