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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Nitrosopumilus maritimus 30S ribosomal protein S17P (rps17p) CSB-YP433403NFB
CSB-EP433403NFB
CSB-BP433403NFB
CSB-MP433403NFB
CSB-EP433403NFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosopumilus maritimus 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP433404NFB
CSB-EP433404NFB
CSB-BP433404NFB
CSB-MP433404NFB
CSB-EP433404NFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 30S ribosomal protein S17e (rps17e) CSB-YP433405MNQ
CSB-EP433405MNQ
CSB-BP433405MNQ
CSB-MP433405MNQ
CSB-EP433405MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP433406MNQ
CSB-EP433406MNQ
CSB-BP433406MNQ
CSB-MP433406MNQ
CSB-EP433406MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0218 protein MmarC6_0469 (MmarC6_0469) CSB-YP433407MNQ
CSB-EP433407MNQ
CSB-BP433407MNQ
CSB-MP433407MNQ
CSB-EP433407MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Putative thymidine phosphorylase (MmarC6_0624), partial CSB-YP433408MNQ
CSB-EP433408MNQ
CSB-BP433408MNQ
CSB-MP433408MNQ
CSB-EP433408MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Phosphoribosylamine--glycine ligase (purD) CSB-YP433409MNQ
CSB-EP433409MNQ
CSB-BP433409MNQ
CSB-MP433409MNQ
CSB-EP433409MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 30S ribosomal protein S2P (rps2p) CSB-YP433410MNQ
CSB-EP433410MNQ
CSB-BP433410MNQ
CSB-MP433410MNQ
CSB-EP433410MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Probable L-aspartate dehydrogenase (nadX) CSB-YP433411MNQ
CSB-EP433411MNQ
CSB-BP433411MNQ
CSB-MP433411MNQ
CSB-EP433411MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP433412MNQ
CSB-EP433412MNQ
CSB-BP433412MNQ
CSB-MP433412MNQ
CSB-EP433412MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Prefoldin subunit beta (pfdB) CSB-YP433413MNQ
CSB-EP433413MNQ
CSB-BP433413MNQ
CSB-MP433413MNQ
CSB-EP433413MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L39e (rpl39e) CSB-YP433414MNQ
CSB-EP433414MNQ
CSB-BP433414MNQ
CSB-MP433414MNQ
CSB-EP433414MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP433415MNQ
CSB-EP433415MNQ
CSB-BP433415MNQ
CSB-MP433415MNQ
CSB-EP433415MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Translation initiation factor 6 (eif6) CSB-YP433416MNQ
CSB-EP433416MNQ
CSB-BP433416MNQ
CSB-MP433416MNQ
CSB-EP433416MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Putative nickel-responsive regulator (MmarC6_0929) CSB-YP433417MNQ
CSB-EP433417MNQ
CSB-BP433417MNQ
CSB-MP433417MNQ
CSB-EP433417MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis DNA polymerase sliding clamp (pcn) CSB-YP433418MNQ
CSB-EP433418MNQ
CSB-BP433418MNQ
CSB-MP433418MNQ
CSB-EP433418MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP433419MNQ
CSB-EP433419MNQ
CSB-BP433419MNQ
CSB-MP433419MNQ
CSB-EP433419MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Nicotinamide-nucleotide adenylyltransferase (MmarC6_1092) CSB-YP433420MNQ
CSB-EP433420MNQ
CSB-BP433420MNQ
CSB-MP433420MNQ
CSB-EP433420MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0212 protein MmarC6_1165 (MmarC6_1165) CSB-YP433421MNQ
CSB-EP433421MNQ
CSB-BP433421MNQ
CSB-MP433421MNQ
CSB-EP433421MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (apgM) CSB-YP433422MNQ
CSB-EP433422MNQ
CSB-BP433422MNQ
CSB-MP433422MNQ
CSB-EP433422MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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