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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Shewanella baltica 30S ribosomal protein S14 (rpsN) CSB-YP434623STN
CSB-EP434623STN
CSB-BP434623STN
CSB-MP434623STN
CSB-EP434623STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 50S ribosomal protein L17 (rplQ) CSB-YP434624STN
CSB-EP434624STN
CSB-BP434624STN
CSB-MP434624STN
CSB-EP434624STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Flagellar L-ring protein (flgH) CSB-YP434625STN
CSB-EP434625STN
CSB-BP434625STN
CSB-MP434625STN
CSB-EP434625STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Probable GTP-binding protein EngB (engB) CSB-YP434626STN
CSB-EP434626STN
CSB-BP434626STN
CSB-MP434626STN
CSB-EP434626STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Membrane protein insertase YidC (yidC), partial CSB-YP434627STN1
CSB-EP434627STN1
CSB-BP434627STN1
CSB-MP434627STN1
CSB-EP434627STN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica tRNA (guanine-N (7)-)-methyltransferase CSB-YP434628STN
CSB-EP434628STN
CSB-BP434628STN
CSB-MP434628STN
CSB-EP434628STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP434629STN
CSB-EP434629STN
CSB-BP434629STN
CSB-MP434629STN
CSB-EP434629STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Protein translocase subunit SecA (secA), partial CSB-YP434630STN
CSB-EP434630STN
CSB-BP434630STN
CSB-MP434630STN
CSB-EP434630STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica UPF0502 protein Sbal195_1802 (Sbal195_1802) CSB-YP434631STN
CSB-EP434631STN
CSB-BP434631STN
CSB-MP434631STN
CSB-EP434631STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP434632STN
CSB-EP434632STN
CSB-BP434632STN
CSB-MP434632STN
CSB-EP434632STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica N utilization substance protein B homolog (nusB) CSB-YP434633STN
CSB-EP434633STN
CSB-BP434633STN
CSB-MP434633STN
CSB-EP434633STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Sec-independent protein translocase protein TatA (tatA), partial CSB-YP434634STN
CSB-EP434634STN
CSB-BP434634STN
CSB-MP434634STN
CSB-EP434634STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica ATP-dependent protease subunit HslV (hslV) CSB-YP434635STN
CSB-EP434635STN
CSB-BP434635STN
CSB-MP434635STN
CSB-EP434635STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Glucans biosynthesis glucosyltransferase H (opgH), partial CSB-YP434636STN1
CSB-EP434636STN1
CSB-BP434636STN1
CSB-MP434636STN1
CSB-EP434636STN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Chaperone protein TorD (torD) CSB-YP434637STN
CSB-EP434637STN
CSB-BP434637STN
CSB-MP434637STN
CSB-EP434637STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 30S ribosomal protein S15 (rpsO) CSB-YP434638STN
CSB-EP434638STN
CSB-BP434638STN
CSB-MP434638STN
CSB-EP434638STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP434639STN
CSB-EP434639STN
CSB-BP434639STN
CSB-MP434639STN
CSB-EP434639STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica UPF0250 protein Sbal195_3458 (Sbal195_3458) CSB-YP434640STN
CSB-EP434640STN
CSB-BP434640STN
CSB-MP434640STN
CSB-EP434640STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Ribonuclease H (rnhA) CSB-YP434641STN
CSB-EP434641STN
CSB-BP434641STN
CSB-MP434641STN
CSB-EP434641STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Ribosomal RNA large subunit methyltransferase G (rlmG) CSB-YP434642STN
CSB-EP434642STN
CSB-BP434642STN
CSB-MP434642STN
CSB-EP434642STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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