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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella paratyphi B Ribosomal RNA large subunit methyltransferase L (rlmL), partial CSB-YP434003STF
CSB-EP434003STF
CSB-BP434003STF
CSB-MP434003STF
CSB-EP434003STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B D-tagatose-1,6-bisphosphate aldolase subunit GatY (gatY) CSB-YP434004STF
CSB-EP434004STF
CSB-BP434004STF
CSB-MP434004STF
CSB-EP434004STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B UPF0213 protein yhbQ (yhbQ) CSB-YP434005STF
CSB-EP434005STF
CSB-BP434005STF
CSB-MP434005STF
CSB-EP434005STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Phosphoglucosamine mutase (glmM) CSB-YP434006STF
CSB-EP434006STF
CSB-BP434006STF
CSB-MP434006STF
CSB-EP434006STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Phosphoglycerol transferase I (mdoB), partial CSB-YP434007STF1
CSB-EP434007STF1
CSB-BP434007STF1
CSB-MP434007STF1
CSB-EP434007STF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP434008STF
CSB-EP434008STF
CSB-BP434008STF
CSB-MP434008STF
CSB-EP434008STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Serine--tRNA ligase (serS) CSB-YP434009STF
CSB-EP434009STF
CSB-BP434009STF
CSB-MP434009STF
CSB-EP434009STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Translation initiation factor IF-1 (infA) CSB-YP434010STF
CSB-EP434010STF
CSB-BP434010STF
CSB-MP434010STF
CSB-EP434010STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B 30S ribosomal protein S9 (rpsI) CSB-YP434011STF
CSB-EP434011STF
CSB-BP434011STF
CSB-MP434011STF
CSB-EP434011STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Protein smg (smg) CSB-YP434012STF
CSB-EP434012STF
CSB-BP434012STF
CSB-MP434012STF
CSB-EP434012STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B 50S ribosomal protein L17 (rplQ) CSB-YP434013STF
CSB-EP434013STF
CSB-BP434013STF
CSB-MP434013STF
CSB-EP434013STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP434014CXH
CSB-EP434014CXH
CSB-BP434014CXH
CSB-MP434014CXH
CSB-EP434014CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP434015CXH
CSB-EP434015CXH
CSB-BP434015CXH
CSB-MP434015CXH
CSB-EP434015CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 3-dehydroquinate dehydratase (aroD) CSB-YP434016CXH
CSB-EP434016CXH
CSB-BP434016CXH
CSB-MP434016CXH
CSB-EP434016CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Protein-export protein secB (secB) CSB-YP434017CXH
CSB-EP434017CXH
CSB-BP434017CXH
CSB-MP434017CXH
CSB-EP434017CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP434018CXH
CSB-EP434018CXH
CSB-BP434018CXH
CSB-MP434018CXH
CSB-EP434018CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Bis (5'-nucleosyl)-tetraphosphatase, symmetrical CSB-YP434019CXH
CSB-EP434019CXH
CSB-BP434019CXH
CSB-MP434019CXH
CSB-EP434019CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 30S ribosomal protein S21 (rpsU) CSB-YP434020CXH
CSB-EP434020CXH
CSB-BP434020CXH
CSB-MP434020CXH
CSB-EP434020CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP434021CXH
CSB-EP434021CXH
CSB-BP434021CXH
CSB-MP434021CXH
CSB-EP434021CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 10 kDa chaperonin (groS) CSB-YP434022CXH
CSB-EP434022CXH
CSB-BP434022CXH
CSB-MP434022CXH
CSB-EP434022CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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