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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Yersinia pestis bv. Antiqua 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP435824YAM
CSB-EP435824YAM
CSB-BP435824YAM
CSB-MP435824YAM
CSB-EP435824YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua RNA polymerase-associated protein RapA (rapA), partial CSB-YP435825YAM
CSB-EP435825YAM
CSB-BP435825YAM
CSB-MP435825YAM
CSB-EP435825YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua DNA gyrase inhibitor YacG (yacG) CSB-YP435826YAM
CSB-EP435826YAM
CSB-BP435826YAM
CSB-MP435826YAM
CSB-EP435826YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Monofunctional biosynthetic peptidoglycan transglycosylase (mtgA), partial CSB-YP435827YAM1
CSB-EP435827YAM1
CSB-BP435827YAM1
CSB-MP435827YAM1
CSB-EP435827YAM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 3-dehydroquinate dehydratase (aroQ) CSB-YP435828YAM
CSB-EP435828YAM
CSB-BP435828YAM
CSB-MP435828YAM
CSB-EP435828YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua LexA repressor (lexA) CSB-YP435829YAM
CSB-EP435829YAM
CSB-BP435829YAM
CSB-MP435829YAM
CSB-EP435829YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua UPF0756 membrane protein YpAngola_A3137 (YpAngola_A3137), partial CSB-YP435830YAM1
CSB-EP435830YAM1
CSB-BP435830YAM1
CSB-MP435830YAM1
CSB-EP435830YAM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Deoxycytidine triphosphate deaminase (dcd) CSB-YP435831YAM
CSB-EP435831YAM
CSB-BP435831YAM
CSB-MP435831YAM
CSB-EP435831YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Ribosomal RNA large subunit methyltransferase I (rlmI) CSB-YP435832YAM
CSB-EP435832YAM
CSB-BP435832YAM
CSB-MP435832YAM
CSB-EP435832YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua RNA pyrophosphohydrolase (rppH) CSB-YP435833YAM
CSB-EP435833YAM
CSB-BP435833YAM
CSB-MP435833YAM
CSB-EP435833YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Shikimate kinase 2 (aroL) CSB-YP435834YAM
CSB-EP435834YAM
CSB-BP435834YAM
CSB-MP435834YAM
CSB-EP435834YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Xanthine phosphoribosyltransferase (gpt) CSB-YP435835YAM
CSB-EP435835YAM
CSB-BP435835YAM
CSB-MP435835YAM
CSB-EP435835YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Putative Holliday junction resolvase (YpAngola_A3340) CSB-YP435836YAM
CSB-EP435836YAM
CSB-BP435836YAM
CSB-MP435836YAM
CSB-EP435836YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua UPF0253 protein YpAngola_A3414 (YpAngola_A3414) CSB-YP435837YAM
CSB-EP435837YAM
CSB-BP435837YAM
CSB-MP435837YAM
CSB-EP435837YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP435838YAM
CSB-EP435838YAM
CSB-BP435838YAM
CSB-MP435838YAM
CSB-EP435838YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP435839YAM
CSB-EP435839YAM
CSB-BP435839YAM
CSB-MP435839YAM
CSB-EP435839YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Nucleoid-associated protein YpAngola_A1486 (YpAngola_A1486) CSB-YP435840YAM
CSB-EP435840YAM
CSB-BP435840YAM
CSB-MP435840YAM
CSB-EP435840YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Urease accessory protein UreE (ureE) CSB-YP435841YAM
CSB-EP435841YAM
CSB-BP435841YAM
CSB-MP435841YAM
CSB-EP435841YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua N-acetylmuramic acid 6-phosphate etherase (murQ) CSB-YP435842YAM
CSB-EP435842YAM
CSB-BP435842YAM
CSB-MP435842YAM
CSB-EP435842YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Protein TusC (tusC) CSB-YP435843YAM
CSB-EP435843YAM
CSB-BP435843YAM
CSB-MP435843YAM
CSB-EP435843YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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