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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Yersinia pestis bv. Antiqua DNA ligase B (ligB), partial CSB-YP434123YAM
CSB-EP434123YAM
CSB-BP434123YAM
CSB-MP434123YAM
CSB-EP434123YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP434124YAM
CSB-EP434124YAM
CSB-BP434124YAM
CSB-MP434124YAM
CSB-EP434124YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP434125YAM
CSB-EP434125YAM
CSB-BP434125YAM
CSB-MP434125YAM
CSB-EP434125YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP434126YAM
CSB-EP434126YAM
CSB-BP434126YAM
CSB-MP434126YAM
CSB-EP434126YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua tRNA (uracil (54)-C (5))-methyltransferase CSB-YP434127YAM
CSB-EP434127YAM
CSB-BP434127YAM
CSB-MP434127YAM
CSB-EP434127YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua LPS-assembly lipoprotein lptE (lptE) CSB-YP434128YAM
CSB-EP434128YAM
CSB-BP434128YAM
CSB-MP434128YAM
CSB-EP434128YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP434129YAM
CSB-EP434129YAM
CSB-BP434129YAM
CSB-MP434129YAM
CSB-EP434129YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP434130YAM
CSB-EP434130YAM
CSB-BP434130YAM
CSB-MP434130YAM
CSB-EP434130YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Chromosome partition protein MukB (mukB), partial CSB-YP434131YAM
CSB-EP434131YAM
CSB-BP434131YAM
CSB-MP434131YAM
CSB-EP434131YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP434132YAM
CSB-EP434132YAM
CSB-BP434132YAM
CSB-MP434132YAM
CSB-EP434132YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua NADH pyrophosphatase (nudC) CSB-YP434133YAM
CSB-EP434133YAM
CSB-BP434133YAM
CSB-MP434133YAM
CSB-EP434133YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Electron transport complex protein RnfB (rnfB) CSB-YP434134YAM
CSB-EP434134YAM
CSB-BP434134YAM
CSB-MP434134YAM
CSB-EP434134YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 50S ribosomal protein L6 (rplF) CSB-YP434135YAM
CSB-EP434135YAM
CSB-BP434135YAM
CSB-MP434135YAM
CSB-EP434135YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 30S ribosomal protein S13 (rpsM) CSB-YP434136YAM
CSB-EP434136YAM
CSB-BP434136YAM
CSB-MP434136YAM
CSB-EP434136YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Large-conductance mechanosensitive channel (mscL), partial CSB-YP434137YAM1
CSB-EP434137YAM1
CSB-BP434137YAM1
CSB-MP434137YAM1
CSB-EP434137YAM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua tRNA threonylcarbamoyladenosine biosynthesis protein RimN (rimN) CSB-YP434138YAM
CSB-EP434138YAM
CSB-BP434138YAM
CSB-MP434138YAM
CSB-EP434138YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Peptide methionine sulfoxide reductase MsrB (msrB) CSB-YP434139YAM
CSB-EP434139YAM
CSB-BP434139YAM
CSB-MP434139YAM
CSB-EP434139YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 (PHYPADRAFT_111231), partial CSB-YP434140EVD
CSB-EP434140EVD
CSB-BP434140EVD
CSB-MP434140EVD
CSB-EP434140EVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens CASP-like protein PHYPADRAFT_73452 (PHYPADRAFT_73452), partial CSB-YP434141EVD1
CSB-EP434141EVD1
CSB-BP434141EVD1
CSB-MP434141EVD1
CSB-EP434141EVD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens Anamorsin homolog 1 (PHYPADRAFT_163171) CSB-YP434142EVD
CSB-EP434142EVD
CSB-BP434142EVD
CSB-MP434142EVD
CSB-EP434142EVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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