Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Acholeplasma laidlawii Non-canonical purine NTP pyrophosphatase (ACL_0869) CSB-YP434923ATX
CSB-EP434923ATX
CSB-BP434923ATX
CSB-MP434923ATX
CSB-EP434923ATX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acholeplasma laidlawii 30S ribosomal protein S21 (rpsU) CSB-YP434924ATX
CSB-EP434924ATX
CSB-BP434924ATX
CSB-MP434924ATX
CSB-EP434924ATX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acholeplasma laidlawii Phosphoglycerate kinase (pgk) CSB-YP434925ATX
CSB-EP434925ATX
CSB-BP434925ATX
CSB-MP434925ATX
CSB-EP434925ATX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picea sitchensis Lipoyl synthase, mitochondrial (LIP1), partial CSB-YP434926EVO
CSB-EP434926EVO
CSB-BP434926EVO
CSB-MP434926EVO
CSB-EP434926EVO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picea sitchensis CASP-like protein 6, partial CSB-YP434927EVO1
CSB-EP434927EVO1
CSB-BP434927EVO1
CSB-MP434927EVO1
CSB-EP434927EVO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Populus trichocarpa S-adenosylmethionine synthase 3 (METK3) CSB-YP434928EXS
CSB-EP434928EXS
CSB-BP434928EXS
CSB-MP434928EXS
CSB-EP434928EXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trachelium caeruleum NAD (P)H-quinone oxidoreductase subunit K, chloroplastic CSB-YP434930TOW
CSB-EP434930TOW
CSB-BP434930TOW
CSB-MP434930TOW
CSB-EP434930TOW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trachelium caeruleum 30S ribosomal protein S15, chloroplastic (rps15-A) CSB-YP434931TOW
CSB-EP434931TOW
CSB-BP434931TOW
CSB-MP434931TOW
CSB-EP434931TOW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua ATPase ravA (ravA), partial CSB-YP434932YAM
CSB-EP434932YAM
CSB-BP434932YAM
CSB-MP434932YAM
CSB-EP434932YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 30S ribosomal protein S6 (rpsF) CSB-YP434933YAM
CSB-EP434933YAM
CSB-BP434933YAM
CSB-MP434933YAM
CSB-EP434933YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Divalent-cation tolerance protein CutA (cutA) CSB-YP434934YAM
CSB-EP434934YAM
CSB-BP434934YAM
CSB-MP434934YAM
CSB-EP434934YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua UPF0260 protein YpAngola_A2394 (YpAngola_A2394) CSB-YP434935YAM
CSB-EP434935YAM
CSB-BP434935YAM
CSB-MP434935YAM
CSB-EP434935YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Spermidine export protein MdtJ (mdtJ), partial CSB-YP434936YAM1
CSB-EP434936YAM1
CSB-BP434936YAM1
CSB-MP434936YAM1
CSB-EP434936YAM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Arginine N-succinyltransferase (astA) CSB-YP434937YAM
CSB-EP434937YAM
CSB-BP434937YAM
CSB-MP434937YAM
CSB-EP434937YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua DNA ligase (ligA), partial CSB-YP434938YAM
CSB-EP434938YAM
CSB-BP434938YAM
CSB-MP434938YAM
CSB-EP434938YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Periplasmic nitrate reductase (napA), partial CSB-YP434939YAM
CSB-EP434939YAM
CSB-BP434939YAM
CSB-MP434939YAM
CSB-EP434939YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua tRNA sulfurtransferase (thiI) CSB-YP434940YAM
CSB-EP434940YAM
CSB-BP434940YAM
CSB-MP434940YAM
CSB-EP434940YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP434941YAM
CSB-EP434941YAM
CSB-BP434941YAM
CSB-MP434941YAM
CSB-EP434941YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Peptide chain release factor 3 (prfC), partial CSB-YP434942YAM
CSB-EP434942YAM
CSB-BP434942YAM
CSB-MP434942YAM
CSB-EP434942YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF (arnF), partial CSB-YP434943YAM1
CSB-EP434943YAM1
CSB-BP434943YAM1
CSB-MP434943YAM1
CSB-EP434943YAM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>