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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus weihenstephanensis Adenylate kinase (adk) CSB-YP434183BON
CSB-EP434183BON
CSB-BP434183BON
CSB-MP434183BON
CSB-EP434183BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Probable D-serine dehydratase (dsdA) CSB-YP434184BON
CSB-EP434184BON
CSB-BP434184BON
CSB-MP434184BON
CSB-EP434184BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis LexA repressor (lexA) CSB-YP434185BON
CSB-EP434185BON
CSB-BP434185BON
CSB-MP434185BON
CSB-EP434185BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis HPr kinase/phosphorylase (hprK) CSB-YP434186BON
CSB-EP434186BON
CSB-BP434186BON
CSB-MP434186BON
CSB-EP434186BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UPF0176 protein BcerKBAB4_1736 (BcerKBAB4_1736) CSB-YP434187BON
CSB-EP434187BON
CSB-BP434187BON
CSB-MP434187BON
CSB-EP434187BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Transcriptional regulator lytR (lytR), partial CSB-YP434188BON
CSB-EP434188BON
CSB-BP434188BON
CSB-MP434188BON
CSB-EP434188BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis DNA ligase (ligA), partial CSB-YP434189BON
CSB-EP434189BON
CSB-BP434189BON
CSB-MP434189BON
CSB-EP434189BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP434190BON
CSB-EP434190BON
CSB-BP434190BON
CSB-MP434190BON
CSB-EP434190BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis NH (3)-dependent NAD (+) synthetase CSB-YP434191BON
CSB-EP434191BON
CSB-BP434191BON
CSB-MP434191BON
CSB-EP434191BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Putative competence-damage inducible protein (cinA) CSB-YP434192BON
CSB-EP434192BON
CSB-BP434192BON
CSB-MP434192BON
CSB-EP434192BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Ribosome-binding factor A (rbfA) CSB-YP434193BON
CSB-EP434193BON
CSB-BP434193BON
CSB-MP434193BON
CSB-EP434193BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis tRNA (guanine-N (1)-)-methyltransferase CSB-YP434194BON
CSB-EP434194BON
CSB-BP434194BON
CSB-MP434194BON
CSB-EP434194BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Phosphate acyltransferase (plsX) CSB-YP434195BON
CSB-EP434195BON
CSB-BP434195BON
CSB-MP434195BON
CSB-EP434195BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Bifunctional protein pyrR (pyrR) CSB-YP434196BON
CSB-EP434196BON
CSB-BP434196BON
CSB-MP434196BON
CSB-EP434196BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Homoserine O-succinyltransferase (metA) CSB-YP434197BON
CSB-EP434197BON
CSB-BP434197BON
CSB-MP434197BON
CSB-EP434197BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Nucleoid occlusion protein (noc) CSB-YP434198BON
CSB-EP434198BON
CSB-BP434198BON
CSB-MP434198BON
CSB-EP434198BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UPF0229 protein BcerKBAB4_0466 (BcerKBAB4_0466) CSB-YP434199BON
CSB-EP434199BON
CSB-BP434199BON
CSB-MP434199BON
CSB-EP434199BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP434200BON
CSB-EP434200BON
CSB-BP434200BON
CSB-MP434200BON
CSB-EP434200BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UPF0298 protein BcerKBAB4_3759 (BcerKBAB4_3759) CSB-YP434201BON
CSB-EP434201BON
CSB-BP434201BON
CSB-MP434201BON
CSB-EP434201BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UPF0358 protein BcerKBAB4_3775 (BcerKBAB4_3775) CSB-YP434202BON
CSB-EP434202BON
CSB-BP434202BON
CSB-MP434202BON
CSB-EP434202BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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