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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella paratyphi B Bifunctional protein aas (aas), partial CSB-YP435724STF1
CSB-EP435724STF1
CSB-BP435724STF1
CSB-MP435724STF1
CSB-EP435724STF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Phosphoglycerate kinase (pgk) CSB-YP435725STF
CSB-EP435725STF
CSB-BP435725STF
CSB-MP435725STF
CSB-EP435725STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B UPF0208 membrane protein YfbV (yfbV), partial CSB-YP435726STF1
CSB-EP435726STF1
CSB-BP435726STF1
CSB-MP435726STF1
CSB-EP435726STF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B UPF0114 protein YqhA (yqhA), partial CSB-YP435727STF1
CSB-EP435727STF1
CSB-BP435727STF1
CSB-MP435727STF1
CSB-EP435727STF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Adenylosuccinate synthetase (purA) CSB-YP435728STF
CSB-EP435728STF
CSB-BP435728STF
CSB-MP435728STF
CSB-EP435728STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP435729STF
CSB-EP435729STF
CSB-BP435729STF
CSB-MP435729STF
CSB-EP435729STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF (arnF), partial CSB-YP435730STF1
CSB-EP435730STF1
CSB-BP435730STF1
CSB-MP435730STF1
CSB-EP435730STF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B 2-keto-3-deoxy-L-rhamnonate aldolase (rhmA) CSB-YP435731STF
CSB-EP435731STF
CSB-BP435731STF
CSB-MP435731STF
CSB-EP435731STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Dihydroorotase (pyrC) CSB-YP435732STF
CSB-EP435732STF
CSB-BP435732STF
CSB-MP435732STF
CSB-EP435732STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Probable phosphatase YcdX (ycdX) CSB-YP435733STF
CSB-EP435733STF
CSB-BP435733STF
CSB-MP435733STF
CSB-EP435733STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B G/U mismatch-specific DNA glycosylase (mug) CSB-YP435734STF
CSB-EP435734STF
CSB-BP435734STF
CSB-MP435734STF
CSB-EP435734STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Aspartate carbamoyltransferase (pyrB) CSB-YP435735STF
CSB-EP435735STF
CSB-BP435735STF
CSB-MP435735STF
CSB-EP435735STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Nucleoid-associated protein YejK (yejK) CSB-YP435736STF
CSB-EP435736STF
CSB-BP435736STF
CSB-MP435736STF
CSB-EP435736STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpcH) CSB-YP435737STF
CSB-EP435737STF
CSB-BP435737STF
CSB-MP435737STF
CSB-EP435737STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B UPF0306 protein yhbP (yhbP) CSB-YP435738STF
CSB-EP435738STF
CSB-BP435738STF
CSB-MP435738STF
CSB-EP435738STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B UPF0391 membrane protein ytjA (ytjA), partial CSB-YP435739STF
CSB-EP435739STF
CSB-BP435739STF
CSB-MP435739STF
CSB-EP435739STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B UPF0387 membrane protein yohO (yohO), partial CSB-YP435740STF
CSB-EP435740STF
CSB-BP435740STF
CSB-MP435740STF
CSB-EP435740STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Probable lipid kinase YegS (yegS) CSB-YP435741STF
CSB-EP435741STF
CSB-BP435741STF
CSB-MP435741STF
CSB-EP435741STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Hydroxylamine reductase (hcp), partial CSB-YP435742STF
CSB-EP435742STF
CSB-BP435742STF
CSB-MP435742STF
CSB-EP435742STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Sulfoxide reductase catalytic subunit YedY (yedY) CSB-YP435743STF
CSB-EP435743STF
CSB-BP435743STF
CSB-MP435743STF
CSB-EP435743STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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