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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Chloroflexus aurantiacus UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP434243DSX
CSB-EP434243DSX
CSB-BP434243DSX
CSB-MP434243DSX
CSB-EP434243DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus 30S ribosomal protein S10 (rpsJ) CSB-YP434244DSX
CSB-EP434244DSX
CSB-BP434244DSX
CSB-MP434244DSX
CSB-EP434244DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus 50S ribosomal protein L5 (rplE) CSB-YP434245DSX
CSB-EP434245DSX
CSB-BP434245DSX
CSB-MP434245DSX
CSB-EP434245DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus 50S ribosomal protein L30 (rpmD) CSB-YP434246DSX
CSB-EP434246DSX
CSB-BP434246DSX
CSB-MP434246DSX
CSB-EP434246DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus Phosphopantetheine adenylyltransferase (coaD) CSB-YP434247DSX
CSB-EP434247DSX
CSB-BP434247DSX
CSB-MP434247DSX
CSB-EP434247DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus Putative pterin-4-alpha-carbinolamine dehydratase (Caur_3165) CSB-YP434248DSX
CSB-EP434248DSX
CSB-BP434248DSX
CSB-MP434248DSX
CSB-EP434248DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP434249DSX
CSB-EP434249DSX
CSB-BP434249DSX
CSB-MP434249DSX
CSB-EP434249DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus Ribonuclease Y (rny), partial CSB-YP434250DSX
CSB-EP434250DSX
CSB-BP434250DSX
CSB-MP434250DSX
CSB-EP434250DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus Sec-independent protein translocase protein TatA (tatA), partial CSB-YP434251DSX1
CSB-EP434251DSX1
CSB-BP434251DSX1
CSB-MP434251DSX1
CSB-EP434251DSX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP434252RIH
CSB-EP434252RIH
CSB-BP434252RIH
CSB-MP434252RIH
CSB-EP434252RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum 30S ribosomal protein S9 (rpsI) CSB-YP434253RIH
CSB-EP434253RIH
CSB-BP434253RIH
CSB-MP434253RIH
CSB-EP434253RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP434254RIH
CSB-EP434254RIH
CSB-BP434254RIH
CSB-MP434254RIH
CSB-EP434254RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum Formate--tetrahydrofolate ligase (fhs), partial CSB-YP434255RIH
CSB-EP434255RIH
CSB-BP434255RIH
CSB-MP434255RIH
CSB-EP434255RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum UPF0182 protein RSal33209_1243 (RSal33209_1243), partial CSB-YP434256RIH
CSB-EP434256RIH
CSB-BP434256RIH
CSB-MP434256RIH
CSB-EP434256RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum SsrA-binding protein (smpB) CSB-YP434257RIH
CSB-EP434257RIH
CSB-BP434257RIH
CSB-MP434257RIH
CSB-EP434257RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum Acetylglutamate kinase (argB) CSB-YP434258RIH
CSB-EP434258RIH
CSB-BP434258RIH
CSB-MP434258RIH
CSB-EP434258RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum 50S ribosomal protein L35 (rpmI) CSB-YP434259RIH
CSB-EP434259RIH
CSB-BP434259RIH
CSB-MP434259RIH
CSB-EP434259RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP434260RIH
CSB-EP434260RIH
CSB-BP434260RIH
CSB-MP434260RIH
CSB-EP434260RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum Protein MraZ (mraZ) CSB-YP434261RIH
CSB-EP434261RIH
CSB-BP434261RIH
CSB-MP434261RIH
CSB-EP434261RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum Aspartate carbamoyltransferase (pyrB) CSB-YP434262RIH
CSB-EP434262RIH
CSB-BP434262RIH
CSB-MP434262RIH
CSB-EP434262RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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