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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xenopus tropicalis UPF0562 protein C7orf55 homolog CSB-YP435884XBF
CSB-EP435884XBF
CSB-BP435884XBF
CSB-MP435884XBF
CSB-EP435884XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Monosiga brevicollis Probable serine incorporator (serinc), partial CSB-YP435885MOD
CSB-EP435885MOD
CSB-BP435885MOD
CSB-MP435885MOD
CSB-EP435885MOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Monosiga brevicollis 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (12562) CSB-YP435886MOD
CSB-EP435886MOD
CSB-BP435886MOD
CSB-MP435886MOD
CSB-EP435886MOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 2,3-diketo-5-methylthiopentyl-1-phosphate enolase (mtnW) CSB-YP435887BON
CSB-EP435887BON
CSB-BP435887BON
CSB-MP435887BON
CSB-EP435887BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Phosphopentomutase (deoB) CSB-YP435888BON
CSB-EP435888BON
CSB-BP435888BON
CSB-MP435888BON
CSB-EP435888BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Riboflavin biosynthesis protein ribBA (ribBA) CSB-YP435889BON
CSB-EP435889BON
CSB-BP435889BON
CSB-MP435889BON
CSB-EP435889BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Elongation factor P (efp) CSB-YP435890BON
CSB-EP435890BON
CSB-BP435890BON
CSB-MP435890BON
CSB-EP435890BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UPF0397 protein BcerKBAB4_2500 (BcerKBAB4_2500), partial CSB-YP435891BON1
CSB-EP435891BON1
CSB-BP435891BON1
CSB-MP435891BON1
CSB-EP435891BON1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Probable endonuclease 4 (nfo) CSB-YP435892BON
CSB-EP435892BON
CSB-BP435892BON
CSB-MP435892BON
CSB-EP435892BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Elongation factor 4 (lepA), partial CSB-YP435893BON
CSB-EP435893BON
CSB-BP435893BON
CSB-MP435893BON
CSB-EP435893BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Glycerol kinase (glpK) CSB-YP435894BON
CSB-EP435894BON
CSB-BP435894BON
CSB-MP435894BON
CSB-EP435894BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 50S ribosomal protein L27 (rpmA) CSB-YP435895BON
CSB-EP435895BON
CSB-BP435895BON
CSB-MP435895BON
CSB-EP435895BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Septum formation protein Maf (maf) CSB-YP435896BON
CSB-EP435896BON
CSB-BP435896BON
CSB-MP435896BON
CSB-EP435896BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UPF0736 protein BcerKBAB4_1085 (BcerKBAB4_1085) CSB-YP435897BON
CSB-EP435897BON
CSB-BP435897BON
CSB-MP435897BON
CSB-EP435897BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Transcriptional repressor NrdR (nrdR) CSB-YP435898BON
CSB-EP435898BON
CSB-BP435898BON
CSB-MP435898BON
CSB-EP435898BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Tryptophan synthase alpha chain (trpA) CSB-YP435899BON
CSB-EP435899BON
CSB-BP435899BON
CSB-MP435899BON
CSB-EP435899BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP435900BON
CSB-EP435900BON
CSB-BP435900BON
CSB-MP435900BON
CSB-EP435900BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Phosphonoacetaldehyde hydrolase (phnX) CSB-YP435901BON
CSB-EP435901BON
CSB-BP435901BON
CSB-MP435901BON
CSB-EP435901BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Probable molybdopterin-guanine dinucleotide biosynthesis protein A (mobA) CSB-YP435902BON
CSB-EP435902BON
CSB-BP435902BON
CSB-MP435902BON
CSB-EP435902BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP435903BON
CSB-EP435903BON
CSB-BP435903BON
CSB-MP435903BON
CSB-EP435903BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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