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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Renibacterium salmoninarum Probable transcriptional regulatory protein RSal33209_2002 (RSal33209_2002) CSB-YP434263RIH
CSB-EP434263RIH
CSB-BP434263RIH
CSB-MP434263RIH
CSB-EP434263RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase (mshC) CSB-YP434264RIH
CSB-EP434264RIH
CSB-BP434264RIH
CSB-MP434264RIH
CSB-EP434264RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum Translation initiation factor IF-1 (infA) CSB-YP434265RIH
CSB-EP434265RIH
CSB-BP434265RIH
CSB-MP434265RIH
CSB-EP434265RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum 50S ribosomal protein L3 (rplC) CSB-YP434266RIH
CSB-EP434266RIH
CSB-BP434266RIH
CSB-MP434266RIH
CSB-EP434266RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP434267RIH
CSB-EP434267RIH
CSB-BP434267RIH
CSB-MP434267RIH
CSB-EP434267RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum Phosphoglycerate kinase (pgk) CSB-YP434268RIH
CSB-EP434268RIH
CSB-BP434268RIH
CSB-MP434268RIH
CSB-EP434268RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum UvrABC system protein B (uvrB) CSB-YP434269RIH
CSB-EP434269RIH
CSB-BP434269RIH
CSB-MP434269RIH
CSB-EP434269RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum Na (+)/H (+) antiporter nhaA, partial CSB-YP434270RIH
CSB-EP434270RIH
CSB-BP434270RIH
CSB-MP434270RIH
CSB-EP434270RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP434271BWU
CSB-EP434271BWU
CSB-BP434271BWU
CSB-MP434271BWU
CSB-EP434271BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis D-aminopeptidase (dap), partial CSB-YP434272BWU
CSB-EP434272BWU
CSB-BP434272BWU
CSB-MP434272BWU
CSB-EP434272BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis Elongation factor 4 (lepA), partial CSB-YP434273BWU
CSB-EP434273BWU
CSB-BP434273BWU
CSB-MP434273BWU
CSB-EP434273BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis Uracil phosphoribosyltransferase (upp) CSB-YP434274BWU
CSB-EP434274BWU
CSB-BP434274BWU
CSB-MP434274BWU
CSB-EP434274BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis Phosphoglucosamine mutase (glmM) CSB-YP434275BWU
CSB-EP434275BWU
CSB-BP434275BWU
CSB-MP434275BWU
CSB-EP434275BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis 50S ribosomal protein L31 (rpmE) CSB-YP434276BWU
CSB-EP434276BWU
CSB-BP434276BWU
CSB-MP434276BWU
CSB-EP434276BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP434277BWU
CSB-EP434277BWU
CSB-BP434277BWU
CSB-MP434277BWU
CSB-EP434277BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis Flagellar hook-basal body complex protein FliE (fliE) CSB-YP434278BWU
CSB-EP434278BWU
CSB-BP434278BWU
CSB-MP434278BWU
CSB-EP434278BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis Glycerol kinase (glpK) CSB-YP434279BWU
CSB-EP434279BWU
CSB-BP434279BWU
CSB-MP434279BWU
CSB-EP434279BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis Blue-light-activated histidine kinase (BSUIS_B0585) CSB-YP434280BWU
CSB-EP434280BWU
CSB-BP434280BWU
CSB-MP434280BWU
CSB-EP434280BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis Alkyl hydroperoxide reductase AhpD (ahpD) CSB-YP434281BWU
CSB-EP434281BWU
CSB-BP434281BWU
CSB-MP434281BWU
CSB-EP434281BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geolycosa sp. Omega-lycotoxin-Gsp2671c CSB-YP434282GEJ
CSB-EP434282GEJ
CSB-BP434282GEJ
CSB-MP434282GEJ
CSB-EP434282GEJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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