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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mouse Lys-63-specific deubiquitinase BRCC36 (Brcc3) CSB-YP002798MO
CSB-BP002798MO
CSB-MP002798MO
CSB-EP002798MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human 60S ribosomal protein L27a (RPL27A) CSB-YP020214HU
CSB-EP020214HU
CSB-BP020214HU
CSB-MP020214HU
CSB-EP020214HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Biliverdin reductase A (Blvra) CSB-YP002721RA
CSB-EP002721RA
CSB-BP002721RA
CSB-MP002721RA
CSB-EP002721RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Yorkie homolog (YAP1) CSB-YP026244CH
CSB-EP026244CH
CSB-BP026244CH
CSB-MP026244CH
CSB-EP026244CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A (STT3A), partial CSB-YP022885HU
CSB-EP022885HU
CSB-BP022885HU
CSB-MP022885HU
CSB-EP022885HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Synaptotagmin-1 (SYT1), partial CSB-YP023029CH1
CSB-EP023029CH1
CSB-BP023029CH1
CSB-MP023029CH1
CSB-EP023029CH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Quinone oxidoreductase (Cryz) CSB-YP006028MO
CSB-EP006028MO
CSB-BP006028MO
CSB-MP006028MO
CSB-EP006028MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Testin (Tes) CSB-YP023392MO
CSB-EP023392MO
CSB-BP023392MO
CSB-MP023392MO
CSB-EP023392MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Alpha-S1-casein (CSN1S1) CSB-YP006060HU
CSB-BP006060HU
CSB-MP006060HU
CSB-EP006060HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Calretinin (Calb2) CSB-YP004433RA
CSB-EP004433RA
CSB-BP004433RA
CSB-MP004433RA
CSB-EP004433RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Aldehyde dehydrogenase, mitochondrial (Aldh2) CSB-YP001571MO
CSB-EP001571MO
CSB-BP001571MO
CSB-MP001571MO
CSB-EP001571MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guinea pig Histamine H2 receptor (HRH2), partial CSB-YP010738GU1
CSB-EP010738GU1
CSB-BP010738GU1
CSB-MP010738GU1
CSB-EP010738GU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse F-actin-capping protein subunit alpha-2 (Capza2) CSB-YP004512MO
CSB-EP004512MO
CSB-BP004512MO
CSB-MP004512MO
CSB-EP004512MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human G-protein coupled receptor 12 (GPR12), partial CSB-YP009742HU1
CSB-EP009742HU1
CSB-BP009742HU1
CSB-MP009742HU1
CSB-EP009742HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Melanocyte-stimulating hormone receptor (MC1R), partial CSB-YP013558BO1
CSB-EP013558BO1
CSB-BP013558BO1
CSB-MP013558BO1
CSB-EP013558BO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Zinc finger protein GLI1 (Gli1), partial CSB-YP009499MO
CSB-EP009499MO
CSB-BP009499MO
CSB-MP009499MO
CSB-EP009499MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Eukaryotic translation initiation factor 1A, X-chromosomal (EIF1AX) CSB-YP007506HU
CSB-BP007506HU
CSB-MP007506HU
CSB-EP007506HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat P2X purinoceptor 1 (P2rx1), partial CSB-YP017319RA
CSB-EP017319RA
CSB-BP017319RA
CSB-MP017319RA
CSB-EP017319RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken 60S ribosomal protein L26 (RPL26) CSB-YP020207CH
CSB-EP020207CH
CSB-BP020207CH
CSB-MP020207CH
CSB-EP020207CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit Matrix Gla protein (MGP) CSB-YP013789RB
CSB-EP013789RB
CSB-BP013789RB
CSB-MP013789RB
CSB-EP013789RB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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