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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mouse Tubulin-specific chaperone A (Tbca) CSB-YP023225MO
CSB-EP023225MO
CSB-BP023225MO
CSB-MP023225MO
CSB-EP023225MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Prospero homeobox protein 1 (Prox1) CSB-YP018757MO
CSB-EP018757MO
CSB-BP018757MO
CSB-MP018757MO
CSB-EP018757MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Serine/threonine-protein phosphatase 2B catalytic subunit gamma isoform (PPP3CC), partial CSB-YP018574HU
CSB-BP018574HU
CSB-MP018574HU
CSB-EP018574HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human G protein-activated inward rectifier potassium channel 4 (KCNJ5), partial CSB-YP012058HU1
CSB-EP012058HU1
CSB-BP012058HU1
CSB-MP012058HU1
CSB-EP012058HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human T-complex protein 1 subunit epsilon (CCT5) CSB-YP004862HU
CSB-EP004862HU
CSB-BP004862HU
CSB-MP004862HU
CSB-EP004862HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Phosphatidylserine synthase 1 (PTDSS1), partial CSB-YP018962HU
CSB-EP018962HU
CSB-BP018962HU
CSB-MP018962HU
CSB-EP018962HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Horse Cytochrome c oxidase subunit 3 (MT-CO3), partial CSB-YP015074HO1
CSB-EP015074HO1
CSB-BP015074HO1
CSB-MP015074HO1
CSB-EP015074HO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Heat shock 70 kDa protein 4L (Hspa4l), partial CSB-YP010826MO
CSB-EP010826MO
CSB-BP010826MO
CSB-MP010826MO
CSB-EP010826MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform (PIK3CG), partial CSB-YP018001HU
CSB-BP018001HU
CSB-MP018001HU
CSB-EP018001HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit Anion exchange protein 2 (SLC4A2), partial CSB-YP021667RB
CSB-EP021667RB
CSB-BP021667RB
CSB-MP021667RB
CSB-EP021667RB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Sodium/hydrogen exchanger 1 (SLC9A1) CSB-YP021726PI
CSB-EP021726PI
CSB-BP021726PI
CSB-MP021726PI
CSB-EP021726PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Deoxycytidine kinase (Dck) CSB-YP006547RA
CSB-EP006547RA
CSB-BP006547RA
CSB-MP006547RA
CSB-EP006547RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Troponin I, fast skeletal muscle (TNNI2) CSB-YP024012HU
CSB-EP024012HU
CSB-BP024012HU
CSB-MP024012HU
CSB-EP024012HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Adhesion G protein-coupled receptor E5 (ADGRE5), partial CSB-YP004972HU1
CSB-EP004972HU1
CSB-BP004972HU1
CSB-MP004972HU1
CSB-EP004972HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Neurogenic differentiation factor 6 (Neurod6) CSB-YP015729MO
CSB-EP015729MO
CSB-BP015729MO
CSB-MP015729MO
CSB-EP015729MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Citron Rho-interacting kinase (Cit), partial CSB-YP005446MO
CSB-EP005446MO
CSB-BP005446MO
CSB-MP005446MO
CSB-EP005446MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Follicle-stimulating hormone receptor (FSHR), partial CSB-YP009021PI1
CSB-EP009021PI1
CSB-BP009021PI1
CSB-MP009021PI1
CSB-EP009021PI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Horse Alpha-fetoprotein (AFP), partial CSB-YP001421HO
CSB-EP001421HO
CSB-BP001421HO
CSB-MP001421HO
CSB-EP001421HO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Nuclear receptor subfamily 2 group C member 2 (NR2C2) CSB-YP016052HU
CSB-EP016052HU
CSB-BP016052HU
CSB-MP016052HU
CSB-EP016052HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse TFIIH basal transcription factor complex helicase XPB subunit (Ercc3) CSB-YP007771MO
CSB-EP007771MO
CSB-BP007771MO
CSB-MP007771MO
CSB-EP007771MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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