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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Prochlorococcus marinus NAD (P)H-quinone oxidoreductase subunit I CSB-YP434383PZC
CSB-EP434383PZC
CSB-BP434383PZC
CSB-MP434383PZC
CSB-EP434383PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP434384PZC
CSB-EP434384PZC
CSB-BP434384PZC
CSB-MP434384PZC
CSB-EP434384PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L10 (rplJ) CSB-YP434385PZC
CSB-EP434385PZC
CSB-BP434385PZC
CSB-MP434385PZC
CSB-EP434385PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Protein psbN (psbN), partial CSB-YP434386PZC
CSB-EP434386PZC
CSB-BP434386PZC
CSB-MP434386PZC
CSB-EP434386PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Orotate phosphoribosyltransferase (pyrE) CSB-YP434387PZC
CSB-EP434387PZC
CSB-BP434387PZC
CSB-MP434387PZC
CSB-EP434387PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus NAD (P)H-quinone oxidoreductase subunit K CSB-YP434388PZC
CSB-EP434388PZC
CSB-BP434388PZC
CSB-MP434388PZC
CSB-EP434388PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem II reaction center protein T (psbT), partial CSB-YP434389PZC
CSB-EP434389PZC
CSB-BP434389PZC
CSB-MP434389PZC
CSB-EP434389PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus GTPase Der (der) CSB-YP434390PZC
CSB-EP434390PZC
CSB-BP434390PZC
CSB-MP434390PZC
CSB-EP434390PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP434391PZC
CSB-EP434391PZC
CSB-BP434391PZC
CSB-MP434391PZC
CSB-EP434391PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP434392PZC
CSB-EP434392PZC
CSB-BP434392PZC
CSB-MP434392PZC
CSB-EP434392PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Light-independent protochlorophyllide reductase iron-sulfur ATP-binding protein (chlL) CSB-YP434393PZC
CSB-EP434393PZC
CSB-BP434393PZC
CSB-MP434393PZC
CSB-EP434393PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem II protein Y (psbY), partial CSB-YP434394PZC
CSB-EP434394PZC
CSB-BP434394PZC
CSB-MP434394PZC
CSB-EP434394PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP434395PYI
CSB-EP434395PYI
CSB-BP434395PYI
CSB-MP434395PYI
CSB-EP434395PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP434396PYI
CSB-EP434396PYI
CSB-BP434396PYI
CSB-MP434396PYI
CSB-EP434396PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Molybdenum cofactor biosynthesis protein A (moaA) CSB-YP434397PYI
CSB-EP434397PYI
CSB-BP434397PYI
CSB-MP434397PYI
CSB-EP434397PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis N-acetylmuramic acid 6-phosphate etherase (murQ) CSB-YP434398PYI
CSB-EP434398PYI
CSB-BP434398PYI
CSB-MP434398PYI
CSB-EP434398PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Translation initiation factor IF-1 (infA) CSB-YP434399PYI
CSB-EP434399PYI
CSB-BP434399PYI
CSB-MP434399PYI
CSB-EP434399PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP434400PYI
CSB-EP434400PYI
CSB-BP434400PYI
CSB-MP434400PYI
CSB-EP434400PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP434401PYI
CSB-EP434401PYI
CSB-BP434401PYI
CSB-MP434401PYI
CSB-EP434401PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis 50S ribosomal protein L28 (rpmB) CSB-YP434402PYI
CSB-EP434402PYI
CSB-BP434402PYI
CSB-MP434402PYI
CSB-EP434402PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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