Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia multivorans D-tyrosyl-tRNA (Tyr) deacylase CSB-YP435184BPS
CSB-EP435184BPS
CSB-BP435184BPS
CSB-MP435184BPS
CSB-EP435184BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP435185BPS1
CSB-EP435185BPS1
CSB-BP435185BPS1
CSB-MP435185BPS1
CSB-EP435185BPS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Putative regulator of ribonuclease activity (Bmul_1223, BMULJ_02024) CSB-YP435186BPS
CSB-EP435186BPS
CSB-BP435186BPS
CSB-MP435186BPS
CSB-EP435186BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Glutamate 5-kinase (proB) CSB-YP435187BPS
CSB-EP435187BPS
CSB-BP435187BPS
CSB-MP435187BPS
CSB-EP435187BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP435188BPS
CSB-EP435188BPS
CSB-BP435188BPS
CSB-MP435188BPS
CSB-EP435188BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP435189BPS
CSB-EP435189BPS
CSB-BP435189BPS
CSB-MP435189BPS
CSB-EP435189BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans LexA repressor (lexA) CSB-YP435190BPS
CSB-EP435190BPS
CSB-BP435190BPS
CSB-MP435190BPS
CSB-EP435190BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Indole-3-glycerol phosphate synthase (trpC) CSB-YP435191BPS
CSB-EP435191BPS
CSB-BP435191BPS
CSB-MP435191BPS
CSB-EP435191BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP435192BPS
CSB-EP435192BPS
CSB-BP435192BPS
CSB-MP435192BPS
CSB-EP435192BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP435193BPS
CSB-EP435193BPS
CSB-BP435193BPS
CSB-MP435193BPS
CSB-EP435193BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 30S ribosomal protein S15 (rpsO) CSB-YP435194BPS
CSB-EP435194BPS
CSB-BP435194BPS
CSB-MP435194BPS
CSB-EP435194BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans 30S ribosomal protein S6 (rpsF) CSB-YP435195BPS
CSB-EP435195BPS
CSB-BP435195BPS
CSB-MP435195BPS
CSB-EP435195BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Ureidoglycolate lyase (Bmul_3283, BMULJ_05242) CSB-YP435196BPS
CSB-EP435196BPS
CSB-BP435196BPS
CSB-MP435196BPS
CSB-EP435196BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans HTH-type transcriptional regulator BetI (betI) CSB-YP435197BPS
CSB-EP435197BPS
CSB-BP435197BPS
CSB-MP435197BPS
CSB-EP435197BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Xylose isomerase (xylA) CSB-YP435198BPS
CSB-EP435198BPS
CSB-BP435198BPS
CSB-MP435198BPS
CSB-EP435198BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus ATP synthase epsilon chain (atpC) CSB-YP435199HSK
CSB-EP435199HSK
CSB-BP435199HSK
CSB-MP435199HSK
CSB-EP435199HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus Glutamate racemase (murI) CSB-YP435200HSK
CSB-EP435200HSK
CSB-BP435200HSK
CSB-MP435200HSK
CSB-EP435200HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus Putative pterin-4-alpha-carbinolamine dehydratase (Haur_2248) CSB-YP435201HSK
CSB-EP435201HSK
CSB-BP435201HSK
CSB-MP435201HSK
CSB-EP435201HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus Urease subunit gamma (ureA) CSB-YP435202HSK
CSB-EP435202HSK
CSB-BP435202HSK
CSB-MP435202HSK
CSB-EP435202HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus 50S ribosomal protein L25 (rplY) CSB-YP435203HSK
CSB-EP435203HSK
CSB-BP435203HSK
CSB-MP435203HSK
CSB-EP435203HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>