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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Acholeplasma laidlawii Enolase (eno) CSB-YP435784ATX
CSB-EP435784ATX
CSB-BP435784ATX
CSB-MP435784ATX
CSB-EP435784ATX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acholeplasma laidlawii Acetate kinase (ackA) CSB-YP435785ATX
CSB-EP435785ATX
CSB-BP435785ATX
CSB-MP435785ATX
CSB-EP435785ATX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acholeplasma laidlawii 50S ribosomal protein L32 (rpmF) CSB-YP435786ATX
CSB-EP435786ATX
CSB-BP435786ATX
CSB-MP435786ATX
CSB-EP435786ATX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acholeplasma laidlawii Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP435787ATX
CSB-EP435787ATX
CSB-BP435787ATX
CSB-MP435787ATX
CSB-EP435787ATX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acholeplasma laidlawii 50S ribosomal protein L35 (rpmI) CSB-YP435788ATX
CSB-EP435788ATX
CSB-BP435788ATX
CSB-MP435788ATX
CSB-EP435788ATX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acholeplasma laidlawii 30S ribosomal protein S20 (rpsT) CSB-YP435789ATX
CSB-EP435789ATX
CSB-BP435789ATX
CSB-MP435789ATX
CSB-EP435789ATX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acholeplasma laidlawii UPF0176 protein ACL_1123 (ACL_1123) CSB-YP435790ATX
CSB-EP435790ATX
CSB-BP435790ATX
CSB-MP435790ATX
CSB-EP435790ATX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acholeplasma laidlawii Tyrosine--tRNA ligase (tyrS) CSB-YP435791ATX
CSB-EP435791ATX
CSB-BP435791ATX
CSB-MP435791ATX
CSB-EP435791ATX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picea sitchensis CASP-like protein 1, partial CSB-YP435792EVO1
CSB-EP435792EVO1
CSB-BP435792EVO1
CSB-MP435792EVO1
CSB-EP435792EVO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picea sitchensis S-adenosylmethionine synthase 1 (METK1) CSB-YP435793EVO
CSB-EP435793EVO
CSB-BP435793EVO
CSB-MP435793EVO
CSB-EP435793EVO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picea sitchensis S-adenosylmethionine synthase 3 (METK3) CSB-YP435794EVO
CSB-EP435794EVO
CSB-BP435794EVO
CSB-MP435794EVO
CSB-EP435794EVO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Populus trichocarpa S-adenosylmethionine synthase 5 (METK5) CSB-YP435795EXS
CSB-EP435795EXS
CSB-BP435795EXS
CSB-MP435795EXS
CSB-EP435795EXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus Non-structural glycoprotein 4, partial CSB-YP435796RIR
CSB-EP435796RIR
CSB-BP435796RIR
CSB-MP435796RIR
CSB-EP435796RIR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trachelium caeruleum Maturase K (matK), partial CSB-YP435797TOW
CSB-EP435797TOW
CSB-BP435797TOW
CSB-MP435797TOW
CSB-EP435797TOW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trachelium caeruleum NAD (P)H-quinone oxidoreductase subunit 3, chloroplastic, partial CSB-YP435798TOW1
CSB-EP435798TOW1
CSB-BP435798TOW1
CSB-MP435798TOW1
CSB-EP435798TOW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lycosa singoriensis Venom allergen 5 CSB-YP435799LRO
CSB-EP435799LRO
CSB-BP435799LRO
CSB-MP435799LRO
CSB-EP435799LRO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Aspartate--ammonia ligase (asnA) CSB-YP435800YAM
CSB-EP435800YAM
CSB-BP435800YAM
CSB-MP435800YAM
CSB-EP435800YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Probable molybdopterin-guanine dinucleotide biosynthesis protein A (mobA) CSB-YP435801YAM
CSB-EP435801YAM
CSB-BP435801YAM
CSB-MP435801YAM
CSB-EP435801YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 50S ribosomal protein L9 (rplI) CSB-YP435802YAM
CSB-EP435802YAM
CSB-BP435802YAM
CSB-MP435802YAM
CSB-EP435802YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Uncharacterized protein YjeA (yjeA) CSB-YP435803YAM
CSB-EP435803YAM
CSB-BP435803YAM
CSB-MP435803YAM
CSB-EP435803YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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