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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella arizonae 50S ribosomal protein L2 (rplB) CSB-YP434783STE
CSB-EP434783STE
CSB-BP434783STE
CSB-MP434783STE
CSB-EP434783STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae 50S ribosomal protein L16 (rplP) CSB-YP434784STE
CSB-EP434784STE
CSB-BP434784STE
CSB-MP434784STE
CSB-EP434784STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae 30S ribosomal protein S8 (rpsH) CSB-YP434785STE
CSB-EP434785STE
CSB-BP434785STE
CSB-MP434785STE
CSB-EP434785STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae tRNA (mo5U34)-methyltransferase CSB-YP434786STE
CSB-EP434786STE
CSB-BP434786STE
CSB-MP434786STE
CSB-EP434786STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae ProP effector (proQ) CSB-YP434787STE
CSB-EP434787STE
CSB-BP434787STE
CSB-MP434787STE
CSB-EP434787STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae UPF0181 protein yoaH (yoaH) CSB-YP434788STE
CSB-EP434788STE
CSB-BP434788STE
CSB-MP434788STE
CSB-EP434788STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Sulfoxide reductase heme-binding subunit YedZ (yedZ), partial CSB-YP434789STE1
CSB-EP434789STE1
CSB-BP434789STE1
CSB-MP434789STE1
CSB-EP434789STE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Putative sialic acid transporter (nanT), partial CSB-YP434790STE
CSB-EP434790STE
CSB-BP434790STE
CSB-MP434790STE
CSB-EP434790STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae UPF0042 nucleotide-binding protein yhbJ (yhbJ) CSB-YP434791STE
CSB-EP434791STE
CSB-BP434791STE
CSB-MP434791STE
CSB-EP434791STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP434792STE
CSB-EP434792STE
CSB-BP434792STE
CSB-MP434792STE
CSB-EP434792STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Proline--tRNA ligase (proS), partial CSB-YP434793STE
CSB-EP434793STE
CSB-BP434793STE
CSB-MP434793STE
CSB-EP434793STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP434794STE
CSB-EP434794STE
CSB-BP434794STE
CSB-MP434794STE
CSB-EP434794STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae [Protein-PII] uridylyltransferase (glnD), partial CSB-YP434795STE
CSB-EP434795STE
CSB-BP434795STE
CSB-MP434795STE
CSB-EP434795STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae UPF0306 protein yhbP (yhbP) CSB-YP434796STE
CSB-EP434796STE
CSB-BP434796STE
CSB-MP434796STE
CSB-EP434796STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP434797STE1
CSB-EP434797STE1
CSB-BP434797STE1
CSB-MP434797STE1
CSB-EP434797STE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Tryptophan synthase alpha chain (trpA) CSB-YP434798STE
CSB-EP434798STE
CSB-BP434798STE
CSB-MP434798STE
CSB-EP434798STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Protein translocase subunit SecA (secA), partial CSB-YP434799STE
CSB-EP434799STE
CSB-BP434799STE
CSB-MP434799STE
CSB-EP434799STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP434800STE
CSB-EP434800STE
CSB-BP434800STE
CSB-MP434800STE
CSB-EP434800STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP434801STE
CSB-EP434801STE
CSB-BP434801STE
CSB-MP434801STE
CSB-EP434801STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae L-carnitine/gamma-butyrobetaine antiporter (caiT), partial CSB-YP434802STE
CSB-EP434802STE
CSB-BP434802STE
CSB-MP434802STE
CSB-EP434802STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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