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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Clostridium phytofermentans Phosphopantetheine adenylyltransferase (coaD) CSB-YP434603CWZ
CSB-EP434603CWZ
CSB-BP434603CWZ
CSB-MP434603CWZ
CSB-EP434603CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP434604CWZ
CSB-EP434604CWZ
CSB-BP434604CWZ
CSB-MP434604CWZ
CSB-EP434604CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP434605CWZ
CSB-EP434605CWZ
CSB-BP434605CWZ
CSB-MP434605CWZ
CSB-EP434605CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans Homoserine O-succinyltransferase (metA) CSB-YP434606CWZ
CSB-EP434606CWZ
CSB-BP434606CWZ
CSB-MP434606CWZ
CSB-EP434606CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans D-galactosyl-beta-1->3-N-acetyl-D-hexosamine phosphorylase Cphy3030 (Cphy_3030), partial CSB-YP434607CWZ
CSB-EP434607CWZ
CSB-BP434607CWZ
CSB-MP434607CWZ
CSB-EP434607CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans Glutamate--tRNA ligase (gltX) CSB-YP434608CWZ
CSB-EP434608CWZ
CSB-BP434608CWZ
CSB-MP434608CWZ
CSB-EP434608CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans HPr kinase/phosphorylase (hprK) CSB-YP434609CWZ
CSB-EP434609CWZ
CSB-BP434609CWZ
CSB-MP434609CWZ
CSB-EP434609CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans Putative sporulation transcription regulator WhiA (whiA) CSB-YP434610CWZ
CSB-EP434610CWZ
CSB-BP434610CWZ
CSB-MP434610CWZ
CSB-EP434610CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans Peptidase T (pepT) CSB-YP434611CWZ
CSB-EP434611CWZ
CSB-BP434611CWZ
CSB-MP434611CWZ
CSB-EP434611CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans 33 kDa chaperonin (hslO) CSB-YP434612CWZ
CSB-EP434612CWZ
CSB-BP434612CWZ
CSB-MP434612CWZ
CSB-EP434612CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Protein grpE (grpE) CSB-YP434613STN
CSB-EP434613STN
CSB-BP434613STN
CSB-MP434613STN
CSB-EP434613STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Tryptophan synthase beta chain (trpB) CSB-YP434614STN
CSB-EP434614STN
CSB-BP434614STN
CSB-MP434614STN
CSB-EP434614STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Chromosomal replication initiator protein DnaA (dnaA) CSB-YP434615STN
CSB-EP434615STN
CSB-BP434615STN
CSB-MP434615STN
CSB-EP434615STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP434616STN
CSB-EP434616STN
CSB-BP434616STN
CSB-MP434616STN
CSB-EP434616STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 30S ribosomal protein S2 (rpsB) CSB-YP434617STN
CSB-EP434617STN
CSB-BP434617STN
CSB-MP434617STN
CSB-EP434617STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Protoheme IX farnesyltransferase 2 (cyoE2), partial CSB-YP434618STN
CSB-EP434618STN
CSB-BP434618STN
CSB-MP434618STN
CSB-EP434618STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Proline--tRNA ligase (proS), partial CSB-YP434619STN
CSB-EP434619STN
CSB-BP434619STN
CSB-MP434619STN
CSB-EP434619STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Selenide, water dikinase (selD) CSB-YP434620STN
CSB-EP434620STN
CSB-BP434620STN
CSB-MP434620STN
CSB-EP434620STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 30S ribosomal protein S12 (rpsL) CSB-YP434621STN
CSB-EP434621STN
CSB-BP434621STN
CSB-MP434621STN
CSB-EP434621STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 30S ribosomal protein S19 (rpsS) CSB-YP434622STN
CSB-EP434622STN
CSB-BP434622STN
CSB-MP434622STN
CSB-EP434622STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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