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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Coxiella burnetii Glutamate--tRNA ligase 1 (gltX1) CSB-YP435404DXO
CSB-EP435404DXO
CSB-BP435404DXO
CSB-MP435404DXO
CSB-EP435404DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP435405DXO
CSB-EP435405DXO
CSB-BP435405DXO
CSB-MP435405DXO
CSB-EP435405DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii tRNA pseudouridine synthase B (truB) CSB-YP435406DXO
CSB-EP435406DXO
CSB-BP435406DXO
CSB-MP435406DXO
CSB-EP435406DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP435407DXO
CSB-EP435407DXO
CSB-BP435407DXO
CSB-MP435407DXO
CSB-EP435407DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Probable GTP-binding protein EngB (engB) CSB-YP435408DXO
CSB-EP435408DXO
CSB-BP435408DXO
CSB-MP435408DXO
CSB-EP435408DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 60 kDa chaperonin (groL), partial CSB-YP435409DXO
CSB-EP435409DXO
CSB-BP435409DXO
CSB-MP435409DXO
CSB-EP435409DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Leucine--tRNA ligase (leuS) CSB-YP435410DXO
CSB-EP435410DXO
CSB-BP435410DXO
CSB-MP435410DXO
CSB-EP435410DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP435411DXO
CSB-EP435411DXO
CSB-BP435411DXO
CSB-MP435411DXO
CSB-EP435411DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 50S ribosomal protein L36 (rpmJ) CSB-YP435412DXO
CSB-EP435412DXO
CSB-BP435412DXO
CSB-MP435412DXO
CSB-EP435412DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 50S ribosomal protein L24 (rplX) CSB-YP435413DXO
CSB-EP435413DXO
CSB-BP435413DXO
CSB-MP435413DXO
CSB-EP435413DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 30S ribosomal protein S3 (rpsC) CSB-YP435414DXO
CSB-EP435414DXO
CSB-BP435414DXO
CSB-MP435414DXO
CSB-EP435414DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP435415DXO
CSB-EP435415DXO
CSB-BP435415DXO
CSB-MP435415DXO
CSB-EP435415DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 50S ribosomal protein L11 (rplK) CSB-YP435416DXO
CSB-EP435416DXO
CSB-BP435416DXO
CSB-MP435416DXO
CSB-EP435416DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Aspartate carbamoyltransferase (pyrB) CSB-YP435417DXO
CSB-EP435417DXO
CSB-BP435417DXO
CSB-MP435417DXO
CSB-EP435417DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP435418DXO
CSB-EP435418DXO
CSB-BP435418DXO
CSB-MP435418DXO
CSB-EP435418DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP435419DXO
CSB-EP435419DXO
CSB-BP435419DXO
CSB-MP435419DXO
CSB-EP435419DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 50S ribosomal protein L21 (rplU) CSB-YP435420DXO
CSB-EP435420DXO
CSB-BP435420DXO
CSB-MP435420DXO
CSB-EP435420DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Protein MraZ (mraZ) CSB-YP435421DXO
CSB-EP435421DXO
CSB-BP435421DXO
CSB-MP435421DXO
CSB-EP435421DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP435422DXO
CSB-EP435422DXO
CSB-BP435422DXO
CSB-MP435422DXO
CSB-EP435422DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Aspartate--tRNA ligase (aspS), partial CSB-YP435423DXO
CSB-EP435423DXO
CSB-BP435423DXO
CSB-MP435423DXO
CSB-EP435423DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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