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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Herpetosiphon aurantiacus 50S ribosomal protein L20 (rplT) CSB-YP435204HSK
CSB-EP435204HSK
CSB-BP435204HSK
CSB-MP435204HSK
CSB-EP435204HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus Deoxycytidine triphosphate deaminase (dcd) CSB-YP435205HSK
CSB-EP435205HSK
CSB-BP435205HSK
CSB-MP435205HSK
CSB-EP435205HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus Phosphoglycerate kinase (pgk) CSB-YP435206HSK
CSB-EP435206HSK
CSB-BP435206HSK
CSB-MP435206HSK
CSB-EP435206HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus Lon protease 2 (lon2), partial CSB-YP435207HSK
CSB-EP435207HSK
CSB-BP435207HSK
CSB-MP435207HSK
CSB-EP435207HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus 50S ribosomal protein L16 (rplP) CSB-YP435208HSK
CSB-EP435208HSK
CSB-BP435208HSK
CSB-MP435208HSK
CSB-EP435208HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus 30S ribosomal protein S11 (rpsK) CSB-YP435209HSK
CSB-EP435209HSK
CSB-BP435209HSK
CSB-MP435209HSK
CSB-EP435209HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus 30S ribosomal protein S18 (rpsR) CSB-YP435210HSK
CSB-EP435210HSK
CSB-BP435210HSK
CSB-MP435210HSK
CSB-EP435210HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus Formate--tetrahydrofolate ligase (fhs), partial CSB-YP435211HSK
CSB-EP435211HSK
CSB-BP435211HSK
CSB-MP435211HSK
CSB-EP435211HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP435212HSK
CSB-EP435212HSK
CSB-BP435212HSK
CSB-MP435212HSK
CSB-EP435212HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus Glutamyl-tRNA reductase (hemA) CSB-YP435213HSK
CSB-EP435213HSK
CSB-BP435213HSK
CSB-MP435213HSK
CSB-EP435213HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus 50S ribosomal protein L7/L12 (rplL) CSB-YP435214HSK
CSB-EP435214HSK
CSB-BP435214HSK
CSB-MP435214HSK
CSB-EP435214HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP435215HSK
CSB-EP435215HSK
CSB-BP435215HSK
CSB-MP435215HSK
CSB-EP435215HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus 60 kDa chaperonin (groL), partial CSB-YP435216HSK
CSB-EP435216HSK
CSB-BP435216HSK
CSB-MP435216HSK
CSB-EP435216HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP435217HSK
CSB-EP435217HSK
CSB-BP435217HSK
CSB-MP435217HSK
CSB-EP435217HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP435218HSK
CSB-EP435218HSK
CSB-BP435218HSK
CSB-MP435218HSK
CSB-EP435218HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP435219HSK
CSB-EP435219HSK
CSB-BP435219HSK
CSB-MP435219HSK
CSB-EP435219HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP435220HSK
CSB-EP435220HSK
CSB-BP435220HSK
CSB-MP435220HSK
CSB-EP435220HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP435221PZC
CSB-EP435221PZC
CSB-BP435221PZC
CSB-MP435221PZC
CSB-EP435221PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP435222PZC
CSB-EP435222PZC
CSB-BP435222PZC
CSB-MP435222PZC
CSB-EP435222PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Leucine--tRNA ligase (leuS), partial CSB-YP435223PZC
CSB-EP435223PZC
CSB-BP435223PZC
CSB-MP435223PZC
CSB-EP435223PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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