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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Monosiga brevicollis Anamorsin homolog (33738) CSB-YP435004MOD
CSB-EP435004MOD
CSB-BP435004MOD
CSB-MP435004MOD
CSB-EP435004MOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Monosiga brevicollis Probable cytosolic iron-sulfur protein assembly protein CIAO1 homolog (30417), partial CSB-YP435005MOD
CSB-EP435005MOD
CSB-BP435005MOD
CSB-MP435005MOD
CSB-EP435005MOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Segregation and condensation protein A (scpA) CSB-YP435006BON
CSB-EP435006BON
CSB-BP435006BON
CSB-MP435006BON
CSB-EP435006BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Potassium-transporting ATPase C chain (kdpC), partial CSB-YP435007BON1
CSB-EP435007BON1
CSB-BP435007BON1
CSB-MP435007BON1
CSB-EP435007BON1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Ribonuclease Z (rnz) CSB-YP435008BON
CSB-EP435008BON
CSB-BP435008BON
CSB-MP435008BON
CSB-EP435008BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UPF0342 protein BcerKBAB4_0767 (BcerKBAB4_0767) CSB-YP435009BON
CSB-EP435009BON
CSB-BP435009BON
CSB-MP435009BON
CSB-EP435009BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis N utilization substance protein B homolog (nusB) CSB-YP435010BON
CSB-EP435010BON
CSB-BP435010BON
CSB-MP435010BON
CSB-EP435010BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Probable glycine dehydrogenase [decarboxylating] subunit 1 (gcvPA) CSB-YP435011BON
CSB-EP435011BON
CSB-BP435011BON
CSB-MP435011BON
CSB-EP435011BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP435012BON
CSB-EP435012BON
CSB-BP435012BON
CSB-MP435012BON
CSB-EP435012BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP435013BON
CSB-EP435013BON
CSB-BP435013BON
CSB-MP435013BON
CSB-EP435013BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Probable septum site-determining protein MinC (minC) CSB-YP435014BON
CSB-EP435014BON
CSB-BP435014BON
CSB-MP435014BON
CSB-EP435014BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UPF0344 protein BcerKBAB4_1054 (BcerKBAB4_1054), partial CSB-YP435015BON1
CSB-EP435015BON1
CSB-BP435015BON1
CSB-MP435015BON1
CSB-EP435015BON1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 50S ribosomal protein L35 (rpmI) CSB-YP435016BON
CSB-EP435016BON
CSB-BP435016BON
CSB-MP435016BON
CSB-EP435016BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UPF0756 membrane protein BcerKBAB4_4425 (BcerKBAB4_4425), partial CSB-YP435017BON1
CSB-EP435017BON1
CSB-BP435017BON1
CSB-MP435017BON1
CSB-EP435017BON1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Anthranilate phosphoribosyltransferase (trpD) CSB-YP435018BON
CSB-EP435018BON
CSB-BP435018BON
CSB-MP435018BON
CSB-EP435018BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Pyrrolidone-carboxylate peptidase (pcp) CSB-YP435019BON
CSB-EP435019BON
CSB-BP435019BON
CSB-MP435019BON
CSB-EP435019BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UPF0354 protein BcerKBAB4_4524 (BcerKBAB4_4524) CSB-YP435020BON
CSB-EP435020BON
CSB-BP435020BON
CSB-MP435020BON
CSB-EP435020BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UPF0180 protein BcerKBAB4_1316 (BcerKBAB4_1316) CSB-YP435021BON
CSB-EP435021BON
CSB-BP435021BON
CSB-MP435021BON
CSB-EP435021BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis S-ribosylhomocysteine lyase (luxS) CSB-YP435022BON
CSB-EP435022BON
CSB-BP435022BON
CSB-MP435022BON
CSB-EP435022BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP435023BON
CSB-EP435023BON
CSB-BP435023BON
CSB-MP435023BON
CSB-EP435023BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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