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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Opitutus terrae Urease subunit gamma (ureA) CSB-YP450215OEN
CSB-EP450215OEN
CSB-BP450215OEN
CSB-MP450215OEN
CSB-EP450215OEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opitutus terrae Ribonuclease Y (rny), partial CSB-YP450216OEN1
CSB-EP450216OEN1
CSB-BP450216OEN1
CSB-MP450216OEN1
CSB-EP450216OEN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opitutus terrae Protein CrcB homolog (crcB), partial CSB-YP450217OEN1
CSB-EP450217OEN1
CSB-BP450217OEN1
CSB-MP450217OEN1
CSB-EP450217OEN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opitutus terrae NADH-quinone oxidoreductase subunit D 1 (nuoD1) CSB-YP450218OEN
CSB-EP450218OEN
CSB-BP450218OEN
CSB-MP450218OEN
CSB-EP450218OEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opitutus terrae 50S ribosomal protein L34 (rpmH) CSB-YP450219OEN
CSB-EP450219OEN
CSB-BP450219OEN
CSB-MP450219OEN
CSB-EP450219OEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opitutus terrae UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP450220OEN
CSB-EP450220OEN
CSB-BP450220OEN
CSB-MP450220OEN
CSB-EP450220OEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opitutus terrae NADH-quinone oxidoreductase subunit N 2 (nuoN2), partial CSB-YP450221OEN
CSB-EP450221OEN
CSB-BP450221OEN
CSB-MP450221OEN
CSB-EP450221OEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opitutus terrae Histidine--tRNA ligase (hisS) CSB-YP450222OEN
CSB-EP450222OEN
CSB-BP450222OEN
CSB-MP450222OEN
CSB-EP450222OEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opitutus terrae Ribosomal RNA large subunit methyltransferase N 2 (rlmN2) CSB-YP450223OEN
CSB-EP450223OEN
CSB-BP450223OEN
CSB-MP450223OEN
CSB-EP450223OEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opitutus terrae Indole-3-glycerol phosphate synthase (trpC) CSB-YP450224OEN
CSB-EP450224OEN
CSB-BP450224OEN
CSB-MP450224OEN
CSB-EP450224OEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opitutus terrae Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP450225OEN
CSB-EP450225OEN
CSB-BP450225OEN
CSB-MP450225OEN
CSB-EP450225OEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opitutus terrae Bifunctional protein FolD (folD) CSB-YP450226OEN
CSB-EP450226OEN
CSB-BP450226OEN
CSB-MP450226OEN
CSB-EP450226OEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opitutus terrae 30S ribosomal protein S16 (rpsP) CSB-YP450227OEN
CSB-EP450227OEN
CSB-BP450227OEN
CSB-MP450227OEN
CSB-EP450227OEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus UPF0251 protein Nther_2417 (Nther_2417) CSB-YP450228NFO
CSB-EP450228NFO
CSB-BP450228NFO
CSB-MP450228NFO
CSB-EP450228NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus Heat-inducible transcription repressor HrcA (hrcA) CSB-YP450229NFO
CSB-EP450229NFO
CSB-BP450229NFO
CSB-MP450229NFO
CSB-EP450229NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus 3-dehydroquinate synthase (aroB) CSB-YP450230NFO
CSB-EP450230NFO
CSB-BP450230NFO
CSB-MP450230NFO
CSB-EP450230NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus Protein MraZ (mraZ) CSB-YP450231NFO
CSB-EP450231NFO
CSB-BP450231NFO
CSB-MP450231NFO
CSB-EP450231NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus 50S ribosomal protein L32 (rpmF) CSB-YP450232NFO
CSB-EP450232NFO
CSB-BP450232NFO
CSB-MP450232NFO
CSB-EP450232NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus Ribosome maturation factor RimM (rimM) CSB-YP450233NFO
CSB-EP450233NFO
CSB-BP450233NFO
CSB-MP450233NFO
CSB-EP450233NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus Dihydroorotate dehydrogenase B (NAD (+)), catalytic subunit CSB-YP450234NFO
CSB-EP450234NFO
CSB-BP450234NFO
CSB-MP450234NFO
CSB-EP450234NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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