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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Synechococcus sp. ATP synthase subunit b 1 (atpF1), partial CSB-YP450015FQA1
CSB-EP450015FQA1
CSB-BP450015FQA1
CSB-MP450015FQA1
CSB-EP450015FQA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Deoxyribose-phosphate aldolase (deoC) CSB-YP450016FQA
CSB-EP450016FQA
CSB-BP450016FQA
CSB-MP450016FQA
CSB-EP450016FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Dihydroorotase (pyrC) CSB-YP450017FQA
CSB-EP450017FQA
CSB-BP450017FQA
CSB-MP450017FQA
CSB-EP450017FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L28 (rpmB) CSB-YP450018FQA
CSB-EP450018FQA
CSB-BP450018FQA
CSB-MP450018FQA
CSB-EP450018FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP450019FQA
CSB-EP450019FQA
CSB-BP450019FQA
CSB-MP450019FQA
CSB-EP450019FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP450020FQA
CSB-EP450020FQA
CSB-BP450020FQA
CSB-MP450020FQA
CSB-EP450020FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 30S ribosomal protein S9 (rpsI) CSB-YP450021FQA
CSB-EP450021FQA
CSB-BP450021FQA
CSB-MP450021FQA
CSB-EP450021FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L6 (rplF) CSB-YP450022FQA
CSB-EP450022FQA
CSB-BP450022FQA
CSB-MP450022FQA
CSB-EP450022FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L14 (rplN) CSB-YP450023FQA
CSB-EP450023FQA
CSB-BP450023FQA
CSB-MP450023FQA
CSB-EP450023FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP450024FQA
CSB-EP450024FQA
CSB-BP450024FQA
CSB-MP450024FQA
CSB-EP450024FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP450025FQA
CSB-EP450025FQA
CSB-BP450025FQA
CSB-MP450025FQA
CSB-EP450025FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. tRNA modification GTPase MnmE (mnmE) CSB-YP450026FQA
CSB-EP450026FQA
CSB-BP450026FQA
CSB-MP450026FQA
CSB-EP450026FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Chromosomal replication initiator protein DnaA (dnaA) CSB-YP450027FQA
CSB-EP450027FQA
CSB-BP450027FQA
CSB-MP450027FQA
CSB-EP450027FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP450028FQA
CSB-EP450028FQA
CSB-BP450028FQA
CSB-MP450028FQA
CSB-EP450028FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Gamma-glutamyl phosphate reductase (proA) CSB-YP450029FQA
CSB-EP450029FQA
CSB-BP450029FQA
CSB-MP450029FQA
CSB-EP450029FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP450030FQA
CSB-EP450030FQA
CSB-BP450030FQA
CSB-MP450030FQA
CSB-EP450030FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP450031FQA
CSB-EP450031FQA
CSB-BP450031FQA
CSB-MP450031FQA
CSB-EP450031FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. UPF0367 protein SYNPCC7002_A0153 (SYNPCC7002_A0153) CSB-YP450032FQA
CSB-EP450032FQA
CSB-BP450032FQA
CSB-MP450032FQA
CSB-EP450032FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. N-acetylmuramic acid 6-phosphate etherase (murQ) CSB-YP450033FQA
CSB-EP450033FQA
CSB-BP450033FQA
CSB-MP450033FQA
CSB-EP450033FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. GTPase obg (obg) CSB-YP450034FQA
CSB-EP450034FQA
CSB-BP450034FQA
CSB-MP450034FQA
CSB-EP450034FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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