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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Exiguobacterium sibiricum 50S ribosomal protein L31 type B (rpmE2) CSB-YP450115EPT
CSB-EP450115EPT
CSB-BP450115EPT
CSB-MP450115EPT
CSB-EP450115EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Adenylosuccinate synthetase (purA) CSB-YP450116EPT
CSB-EP450116EPT
CSB-BP450116EPT
CSB-MP450116EPT
CSB-EP450116EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP450117EPT
CSB-EP450117EPT
CSB-BP450117EPT
CSB-MP450117EPT
CSB-EP450117EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Bifunctional protein GlmU (glmU) CSB-YP450118EPT
CSB-EP450118EPT
CSB-BP450118EPT
CSB-MP450118EPT
CSB-EP450118EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP450119EPT
CSB-EP450119EPT
CSB-BP450119EPT
CSB-MP450119EPT
CSB-EP450119EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum 50S ribosomal protein L2 (rplB) CSB-YP450120EPT
CSB-EP450120EPT
CSB-BP450120EPT
CSB-MP450120EPT
CSB-EP450120EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum 50S ribosomal protein L24 (rplX) CSB-YP450121EPT
CSB-EP450121EPT
CSB-BP450121EPT
CSB-MP450121EPT
CSB-EP450121EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Adenylate kinase (adk) CSB-YP450122EPT
CSB-EP450122EPT
CSB-BP450122EPT
CSB-MP450122EPT
CSB-EP450122EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Mannitol-1-phosphate 5-dehydrogenase (mtlD) CSB-YP450123EPT
CSB-EP450123EPT
CSB-BP450123EPT
CSB-MP450123EPT
CSB-EP450123EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Chorismate synthase (aroC) CSB-YP450124EPT
CSB-EP450124EPT
CSB-BP450124EPT
CSB-MP450124EPT
CSB-EP450124EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum 50S ribosomal protein L19 (rplS) CSB-YP450125EPT
CSB-EP450125EPT
CSB-BP450125EPT
CSB-MP450125EPT
CSB-EP450125EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Transcription factor fapR (fapR) CSB-YP450126EPT
CSB-EP450126EPT
CSB-BP450126EPT
CSB-MP450126EPT
CSB-EP450126EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Cell division protein DivIB (divIB), partial CSB-YP450127EPT
CSB-EP450127EPT
CSB-BP450127EPT
CSB-MP450127EPT
CSB-EP450127EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Phosphopantetheine adenylyltransferase (coaD) CSB-YP450128EPT
CSB-EP450128EPT
CSB-BP450128EPT
CSB-MP450128EPT
CSB-EP450128EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase (mtnX) CSB-YP450129EPT
CSB-EP450129EPT
CSB-BP450129EPT
CSB-MP450129EPT
CSB-EP450129EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Heptaprenylglyceryl phosphate synthase (pcrB) CSB-YP450130EPT
CSB-EP450130EPT
CSB-BP450130EPT
CSB-MP450130EPT
CSB-EP450130EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum N-acetyldiaminopimelate deacetylase (Exig_2019) CSB-YP450131EPT
CSB-EP450131EPT
CSB-BP450131EPT
CSB-MP450131EPT
CSB-EP450131EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Aspartate--tRNA ligase (aspS), partial CSB-YP450132EPT
CSB-EP450132EPT
CSB-BP450132EPT
CSB-MP450132EPT
CSB-EP450132EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP450133EPT
CSB-EP450133EPT
CSB-BP450133EPT
CSB-MP450133EPT
CSB-EP450133EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Glutamate racemase (murI) CSB-YP450134EPT
CSB-EP450134EPT
CSB-BP450134EPT
CSB-MP450134EPT
CSB-EP450134EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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