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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella paratyphi B Autoinducer 2-binding protein lsrB (lsrB) CSB-YP434843STF
CSB-EP434843STF
CSB-BP434843STF
CSB-MP434843STF
CSB-EP434843STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP434844STF
CSB-EP434844STF
CSB-BP434844STF
CSB-MP434844STF
CSB-EP434844STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B DNA gyrase inhibitor YacG (yacG) CSB-YP434845STF
CSB-EP434845STF
CSB-BP434845STF
CSB-MP434845STF
CSB-EP434845STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B DNA replication terminus site-binding protein (tus) CSB-YP434846STF
CSB-EP434846STF
CSB-BP434846STF
CSB-MP434846STF
CSB-EP434846STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Protein RecA (recA) CSB-YP434847STF
CSB-EP434847STF
CSB-BP434847STF
CSB-MP434847STF
CSB-EP434847STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Pantothenate kinase (coaA) CSB-YP434848STF
CSB-EP434848STF
CSB-BP434848STF
CSB-MP434848STF
CSB-EP434848STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B NADH pyrophosphatase (nudC) CSB-YP434849STF
CSB-EP434849STF
CSB-BP434849STF
CSB-MP434849STF
CSB-EP434849STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Isocitrate dehydrogenase kinase/phosphatase (aceK), partial CSB-YP434850STF
CSB-EP434850STF
CSB-BP434850STF
CSB-MP434850STF
CSB-EP434850STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP434851STF
CSB-EP434851STF
CSB-BP434851STF
CSB-MP434851STF
CSB-EP434851STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Proline--tRNA ligase (proS), partial CSB-YP434852STF
CSB-EP434852STF
CSB-BP434852STF
CSB-MP434852STF
CSB-EP434852STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B HTH-type transcriptional repressor PurR (purR) CSB-YP434853STF
CSB-EP434853STF
CSB-BP434853STF
CSB-MP434853STF
CSB-EP434853STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Peptidase E (pepE) CSB-YP434854STF
CSB-EP434854STF
CSB-BP434854STF
CSB-MP434854STF
CSB-EP434854STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Chorismate--pyruvate lyase (ubiC) CSB-YP434855STF
CSB-EP434855STF
CSB-BP434855STF
CSB-MP434855STF
CSB-EP434855STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B DNA repair protein recO (recO) CSB-YP434856STF
CSB-EP434856STF
CSB-BP434856STF
CSB-MP434856STF
CSB-EP434856STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Chaperone protein hscA (hscA), partial CSB-YP434857STF
CSB-EP434857STF
CSB-BP434857STF
CSB-MP434857STF
CSB-EP434857STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B 50S ribosomal protein L20 (rplT) CSB-YP434858STF
CSB-EP434858STF
CSB-BP434858STF
CSB-MP434858STF
CSB-EP434858STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B UPF0229 protein yeaH (yeaH) CSB-YP434859STF
CSB-EP434859STF
CSB-BP434859STF
CSB-MP434859STF
CSB-EP434859STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Cell division protein FtsB (ftsB), partial CSB-YP434860STF
CSB-EP434860STF
CSB-BP434860STF
CSB-MP434860STF
CSB-EP434860STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Phosphoadenosine phosphosulfate reductase (cysH) CSB-YP434861STF
CSB-EP434861STF
CSB-BP434861STF
CSB-MP434861STF
CSB-EP434861STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP434862STF
CSB-EP434862STF
CSB-BP434862STF
CSB-MP434862STF
CSB-EP434862STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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