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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus weihenstephanensis UPF0447 protein BcerKBAB4_5181 (BcerKBAB4_5181) CSB-YP435044BON
CSB-EP435044BON
CSB-BP435044BON
CSB-MP435044BON
CSB-EP435044BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Glutamate-1-semialdehyde 2,1-aminomutase 1 (hemL1) CSB-YP435045BON
CSB-EP435045BON
CSB-BP435045BON
CSB-MP435045BON
CSB-EP435045BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP435046BON
CSB-EP435046BON
CSB-BP435046BON
CSB-MP435046BON
CSB-EP435046BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 30S ribosomal protein S16 (rpsP) CSB-YP435047BON
CSB-EP435047BON
CSB-BP435047BON
CSB-MP435047BON
CSB-EP435047BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Orotate phosphoribosyltransferase (pyrE) CSB-YP435048BON
CSB-EP435048BON
CSB-BP435048BON
CSB-MP435048BON
CSB-EP435048BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Dihydroorotase (pyrC) CSB-YP435049BON
CSB-EP435049BON
CSB-BP435049BON
CSB-MP435049BON
CSB-EP435049BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP435050BON
CSB-EP435050BON
CSB-BP435050BON
CSB-MP435050BON
CSB-EP435050BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP435051BON
CSB-EP435051BON
CSB-BP435051BON
CSB-MP435051BON
CSB-EP435051BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Uncharacterized N-acetyltransferase BcerKBAB4_3748 (BcerKBAB4_3748) CSB-YP435052BON
CSB-EP435052BON
CSB-BP435052BON
CSB-MP435052BON
CSB-EP435052BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UPF0356 protein BcerKBAB4_3803 (BcerKBAB4_3803) CSB-YP435053BON
CSB-EP435053BON
CSB-BP435053BON
CSB-MP435053BON
CSB-EP435053BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP435054MNY
CSB-EP435054MNY
CSB-BP435054MNY
CSB-MP435054MNY
CSB-EP435054MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP435055MNY
CSB-EP435055MNY
CSB-BP435055MNY
CSB-MP435055MNY
CSB-EP435055MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens Flavoprotein wrbA (wrbA) CSB-YP435056MNY
CSB-EP435056MNY
CSB-BP435056MNY
CSB-MP435056MNY
CSB-EP435056MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP435057MNY
CSB-EP435057MNY
CSB-BP435057MNY
CSB-MP435057MNY
CSB-EP435057MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP435058MNY
CSB-EP435058MNY
CSB-BP435058MNY
CSB-MP435058MNY
CSB-EP435058MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP435059MNY
CSB-EP435059MNY
CSB-BP435059MNY
CSB-MP435059MNY
CSB-EP435059MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP435060MNY
CSB-EP435060MNY
CSB-BP435060MNY
CSB-MP435060MNY
CSB-EP435060MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens Phosphopentomutase (deoB) CSB-YP435061MNY
CSB-EP435061MNY
CSB-BP435061MNY
CSB-MP435061MNY
CSB-EP435061MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens 30S ribosomal protein S2 (rpsB) CSB-YP435062MNY
CSB-EP435062MNY
CSB-BP435062MNY
CSB-MP435062MNY
CSB-EP435062MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens 30S ribosomal protein S12 (rpsL) CSB-YP435063MNY
CSB-EP435063MNY
CSB-BP435063MNY
CSB-MP435063MNY
CSB-EP435063MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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