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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Synechococcus sp. Biotin synthase (bioB) CSB-YP450035FQA
CSB-EP450035FQA
CSB-BP450035FQA
CSB-MP450035FQA
CSB-EP450035FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP450036FQA
CSB-EP450036FQA
CSB-BP450036FQA
CSB-MP450036FQA
CSB-EP450036FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 30S ribosomal protein S6 (rpsF) CSB-YP450037FQA
CSB-EP450037FQA
CSB-BP450037FQA
CSB-MP450037FQA
CSB-EP450037FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP450038FQA
CSB-EP450038FQA
CSB-BP450038FQA
CSB-MP450038FQA
CSB-EP450038FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 5'-nucleotidase surE (surE) CSB-YP450039FQA
CSB-EP450039FQA
CSB-BP450039FQA
CSB-MP450039FQA
CSB-EP450039FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP450040FQA
CSB-EP450040FQA
CSB-BP450040FQA
CSB-MP450040FQA
CSB-EP450040FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Protein translocase subunit SecF (secF), partial CSB-YP450041FQA1
CSB-EP450041FQA1
CSB-BP450041FQA1
CSB-MP450041FQA1
CSB-EP450041FQA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Cell division protein SepF (sepF) CSB-YP450042FQA
CSB-EP450042FQA
CSB-BP450042FQA
CSB-MP450042FQA
CSB-EP450042FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 30S ribosomal protein S20 (rpsT) CSB-YP450043EXM
CSB-EP450043EXM
CSB-BP450043EXM
CSB-MP450043EXM
CSB-EP450043EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP450044EXM
CSB-EP450044EXM
CSB-BP450044EXM
CSB-MP450044EXM
CSB-EP450044EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 50S ribosomal protein L1 (rplA) CSB-YP450045EXM
CSB-EP450045EXM
CSB-BP450045EXM
CSB-MP450045EXM
CSB-EP450045EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Protease HtpX homolog (htpX), partial CSB-YP450046EXM1
CSB-EP450046EXM1
CSB-BP450046EXM1
CSB-MP450046EXM1
CSB-EP450046EXM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 30S ribosomal protein S12 (rpsL) CSB-YP450047EXM
CSB-EP450047EXM
CSB-BP450047EXM
CSB-MP450047EXM
CSB-EP450047EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 50S ribosomal protein L2 (rplB) CSB-YP450048EXM
CSB-EP450048EXM
CSB-BP450048EXM
CSB-MP450048EXM
CSB-EP450048EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 50S ribosomal protein L5 (rplE) CSB-YP450049EXM
CSB-EP450049EXM
CSB-BP450049EXM
CSB-MP450049EXM
CSB-EP450049EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius 30S ribosomal protein S13 (rpsM) CSB-YP450050EXM
CSB-EP450050EXM
CSB-BP450050EXM
CSB-MP450050EXM
CSB-EP450050EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP450051EXM
CSB-EP450051EXM
CSB-BP450051EXM
CSB-MP450051EXM
CSB-EP450051EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius D-alanine--D-alanine ligase (ddl) CSB-YP450052EXM
CSB-EP450052EXM
CSB-BP450052EXM
CSB-MP450052EXM
CSB-EP450052EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius RNA pyrophosphohydrolase (rppH) CSB-YP450053EXM
CSB-EP450053EXM
CSB-BP450053EXM
CSB-MP450053EXM
CSB-EP450053EXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polynucleobacter necessarius Undecaprenyl-diphosphatase (uppP), partial CSB-YP450054EXM1
CSB-EP450054EXM1
CSB-BP450054EXM1
CSB-MP450054EXM1
CSB-EP450054EXM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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