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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Yersinia pestis bv. Antiqua Thiosulfate sulfurtransferase glpE (glpE) CSB-YP435844YAM
CSB-EP435844YAM
CSB-BP435844YAM
CSB-MP435844YAM
CSB-EP435844YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Small heat shock protein ibpB (ibpB) CSB-YP435845YAM
CSB-EP435845YAM
CSB-BP435845YAM
CSB-MP435845YAM
CSB-EP435845YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 50S ribosomal protein L31 (rpmE) CSB-YP435846YAM
CSB-EP435846YAM
CSB-BP435846YAM
CSB-MP435846YAM
CSB-EP435846YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Lysine--tRNA ligase (lysS), partial CSB-YP435847YAM
CSB-EP435847YAM
CSB-BP435847YAM
CSB-MP435847YAM
CSB-EP435847YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP435848YAM
CSB-EP435848YAM
CSB-BP435848YAM
CSB-MP435848YAM
CSB-EP435848YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Acetylglutamate kinase (argB) CSB-YP435849YAM
CSB-EP435849YAM
CSB-BP435849YAM
CSB-MP435849YAM
CSB-EP435849YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP435850YAM
CSB-EP435850YAM
CSB-BP435850YAM
CSB-MP435850YAM
CSB-EP435850YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua UPF0306 protein YpAngola_A4021 (YpAngola_A4021) CSB-YP435851YAM
CSB-EP435851YAM
CSB-BP435851YAM
CSB-MP435851YAM
CSB-EP435851YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Glycogen synthase (glgA) CSB-YP435852YAM
CSB-EP435852YAM
CSB-BP435852YAM
CSB-MP435852YAM
CSB-EP435852YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua tRNA modification GTPase MnmE (mnmE) CSB-YP435853YAM
CSB-EP435853YAM
CSB-BP435853YAM
CSB-MP435853YAM
CSB-EP435853YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP435854YAM
CSB-EP435854YAM
CSB-BP435854YAM
CSB-MP435854YAM
CSB-EP435854YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Nucleoid occlusion factor SlmA (slmA) CSB-YP435855YAM
CSB-EP435855YAM
CSB-BP435855YAM
CSB-MP435855YAM
CSB-EP435855YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Protein-export protein secB (secB) CSB-YP435856YAM
CSB-EP435856YAM
CSB-BP435856YAM
CSB-MP435856YAM
CSB-EP435856YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Regulator of ribonuclease activity A (rraA) CSB-YP435857YAM
CSB-EP435857YAM
CSB-BP435857YAM
CSB-MP435857YAM
CSB-EP435857YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Ribosomal RNA large subunit methyltransferase F (rlmF) CSB-YP435858YAM
CSB-EP435858YAM
CSB-BP435858YAM
CSB-MP435858YAM
CSB-EP435858YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua NADH-quinone oxidoreductase subunit A (nuoA), partial CSB-YP435859YAM1
CSB-EP435859YAM1
CSB-BP435859YAM1
CSB-MP435859YAM1
CSB-EP435859YAM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Flagellar L-ring protein (flgH) CSB-YP435860YAM
CSB-EP435860YAM
CSB-BP435860YAM
CSB-MP435860YAM
CSB-EP435860YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP435861YAM
CSB-EP435861YAM
CSB-BP435861YAM
CSB-MP435861YAM
CSB-EP435861YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Multifunctional CCA protein (cca) CSB-YP435862YAM
CSB-EP435862YAM
CSB-BP435862YAM
CSB-MP435862YAM
CSB-EP435862YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Ribosomal RNA large subunit methyltransferase L (rlmL), partial CSB-YP435863YAM
CSB-EP435863YAM
CSB-BP435863YAM
CSB-MP435863YAM
CSB-EP435863YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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