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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xanthomonas oryzae pv. oryzae Fe/S biogenesis protein NfuA (nfuA) CSB-YP450836XBC
CSB-EP450836XBC
CSB-BP450836XBC
CSB-MP450836XBC
CSB-EP450836XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Cell division protein FtsB (ftsB), partial CSB-YP450837XBC
CSB-EP450837XBC
CSB-BP450837XBC
CSB-MP450837XBC
CSB-EP450837XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae 50S ribosomal protein L20 (rplT) CSB-YP450838XBC
CSB-EP450838XBC
CSB-BP450838XBC
CSB-MP450838XBC
CSB-EP450838XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae 50S ribosomal protein L34 (rpmH) CSB-YP450839XBC
CSB-EP450839XBC
CSB-BP450839XBC
CSB-MP450839XBC
CSB-EP450839XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Putative reductase PXO_03458 (PXO_03458) CSB-YP450840XBC
CSB-EP450840XBC
CSB-BP450840XBC
CSB-MP450840XBC
CSB-EP450840XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP450841XBC
CSB-EP450841XBC
CSB-BP450841XBC
CSB-MP450841XBC
CSB-EP450841XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Phosphoglucosamine mutase (glmM) CSB-YP450842XBC
CSB-EP450842XBC
CSB-BP450842XBC
CSB-MP450842XBC
CSB-EP450842XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Chorismate synthase (aroC) CSB-YP450843XBC
CSB-EP450843XBC
CSB-BP450843XBC
CSB-MP450843XBC
CSB-EP450843XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Arginine--tRNA ligase (argS), partial CSB-YP450844BXU
CSB-EP450844BXU
CSB-BP450844BXU
CSB-MP450844BXU
CSB-EP450844BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP450845BXU
CSB-EP450845BXU
CSB-BP450845BXU
CSB-MP450845BXU
CSB-EP450845BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP450846BXU
CSB-EP450846BXU
CSB-BP450846BXU
CSB-MP450846BXU
CSB-EP450846BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Heat-inducible transcription repressor HrcA (hrcA) CSB-YP450847BXU
CSB-EP450847BXU
CSB-BP450847BXU
CSB-MP450847BXU
CSB-EP450847BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Protein grpE (grpE) CSB-YP450848BXU
CSB-EP450848BXU
CSB-BP450848BXU
CSB-MP450848BXU
CSB-EP450848BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans 50S ribosomal protein L25 (rplY) CSB-YP450849BXU
CSB-EP450849BXU
CSB-BP450849BXU
CSB-MP450849BXU
CSB-EP450849BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP450850BXU
CSB-EP450850BXU
CSB-BP450850BXU
CSB-MP450850BXU
CSB-EP450850BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Enolase (eno) CSB-YP450851BXU
CSB-EP450851BXU
CSB-BP450851BXU
CSB-MP450851BXU
CSB-EP450851BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans UPF0234 protein Bphyt_3208 (Bphyt_3208) CSB-YP450852BXU
CSB-EP450852BXU
CSB-BP450852BXU
CSB-MP450852BXU
CSB-EP450852BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP450853BXU
CSB-EP450853BXU
CSB-BP450853BXU
CSB-MP450853BXU
CSB-EP450853BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans D-tyrosyl-tRNA (Tyr) deacylase CSB-YP450854BXU
CSB-EP450854BXU
CSB-BP450854BXU
CSB-MP450854BXU
CSB-EP450854BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Glutamate 5-kinase (proB) CSB-YP450855BXU
CSB-EP450855BXU
CSB-BP450855BXU
CSB-MP450855BXU
CSB-EP450855BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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