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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Methanococcus maripaludis Glycine--tRNA ligase (glyS), partial CSB-YP435144MNQ
CSB-EP435144MNQ
CSB-BP435144MNQ
CSB-MP435144MNQ
CSB-EP435144MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP435145MNQ
CSB-EP435145MNQ
CSB-BP435145MNQ
CSB-MP435145MNQ
CSB-EP435145MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Acetylglutamate kinase (argB) CSB-YP435146MNQ
CSB-EP435146MNQ
CSB-BP435146MNQ
CSB-MP435146MNQ
CSB-EP435146MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Ribosome biogenesis protein Nop10 (MmarC6_0965) CSB-YP435147MNQ
CSB-EP435147MNQ
CSB-BP435147MNQ
CSB-MP435147MNQ
CSB-EP435147MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 30S ribosomal protein S8e (rps8e) CSB-YP435148MNQ
CSB-EP435148MNQ
CSB-BP435148MNQ
CSB-MP435148MNQ
CSB-EP435148MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Pyruvoyl-dependent arginine decarboxylase (pdaD) CSB-YP435149MNQ
CSB-EP435149MNQ
CSB-BP435149MNQ
CSB-MP435149MNQ
CSB-EP435149MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 5-methylthioadenosine/S-adenosylhomocysteine deaminase (mtaD) CSB-YP435150MNQ
CSB-EP435150MNQ
CSB-BP435150MNQ
CSB-MP435150MNQ
CSB-EP435150MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Adenylosuccinate synthetase (purA) CSB-YP435151MNQ
CSB-EP435151MNQ
CSB-BP435151MNQ
CSB-MP435151MNQ
CSB-EP435151MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 30S ribosomal protein S8P (rps8p) CSB-YP435152MNQ
CSB-EP435152MNQ
CSB-BP435152MNQ
CSB-MP435152MNQ
CSB-EP435152MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 3-dehydroquinate dehydratase (aroD) CSB-YP435153MNQ
CSB-EP435153MNQ
CSB-BP435153MNQ
CSB-MP435153MNQ
CSB-EP435153MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 30S ribosomal protein S7P (rps7p) CSB-YP435154MNQ
CSB-EP435154MNQ
CSB-BP435154MNQ
CSB-MP435154MNQ
CSB-EP435154MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis IMP cyclohydrolase (purO) CSB-YP435155MNQ
CSB-EP435155MNQ
CSB-BP435155MNQ
CSB-MP435155MNQ
CSB-EP435155MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis O-phospho-L-seryl-tRNA:Cys-tRNA synthase (MmarC6_1433) CSB-YP435156MNQ
CSB-EP435156MNQ
CSB-BP435156MNQ
CSB-MP435156MNQ
CSB-EP435156MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Translation initiation factor 2 subunit gamma (eIF2G) CSB-YP435157MNQ
CSB-EP435157MNQ
CSB-BP435157MNQ
CSB-MP435157MNQ
CSB-EP435157MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Hydroxyethylthiazole kinase (thiM) CSB-YP435158MNQ
CSB-EP435158MNQ
CSB-BP435158MNQ
CSB-MP435158MNQ
CSB-EP435158MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis V-type ATP synthase subunit D (atpD) CSB-YP435159MNQ
CSB-EP435159MNQ
CSB-BP435159MNQ
CSB-MP435159MNQ
CSB-EP435159MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Glutamate--tRNA ligase (gltX), partial CSB-YP435160MNQ
CSB-EP435160MNQ
CSB-BP435160MNQ
CSB-MP435160MNQ
CSB-EP435160MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis F420-0:gamma-glutamyl ligase (cofE) CSB-YP435161MNQ
CSB-EP435161MNQ
CSB-BP435161MNQ
CSB-MP435161MNQ
CSB-EP435161MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Diaminopimelate epimerase (dapF) CSB-YP435162MNQ
CSB-EP435162MNQ
CSB-BP435162MNQ
CSB-MP435162MNQ
CSB-EP435162MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans UPF0271 protein Bmul_3034/BMULJ_00198 (Bmul_3034, BMULJ_00198) CSB-YP435163BPS
CSB-EP435163BPS
CSB-BP435163BPS
CSB-MP435163BPS
CSB-EP435163BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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