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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bordetella petrii UPF0178 protein Bpet3884 (Bpet3884) CSB-YP435344BPB
CSB-EP435344BPB
CSB-BP435344BPB
CSB-MP435344BPB
CSB-EP435344BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP435345BPB
CSB-EP435345BPB
CSB-BP435345BPB
CSB-MP435345BPB
CSB-EP435345BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii 10 kDa chaperonin (groS) CSB-YP435346BPB
CSB-EP435346BPB
CSB-BP435346BPB
CSB-MP435346BPB
CSB-EP435346BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii 2-nonaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (coq7) CSB-YP435347BPB
CSB-EP435347BPB
CSB-BP435347BPB
CSB-MP435347BPB
CSB-EP435347BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP435348BPB
CSB-EP435348BPB
CSB-BP435348BPB
CSB-MP435348BPB
CSB-EP435348BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Carbon storage regulator homolog (csrA) CSB-YP435349BPB
CSB-EP435349BPB
CSB-BP435349BPB
CSB-MP435349BPB
CSB-EP435349BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Glutamate 5-kinase (proB) CSB-YP435350BPB
CSB-EP435350BPB
CSB-BP435350BPB
CSB-MP435350BPB
CSB-EP435350BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Chaperone protein DnaK (dnaK), partial CSB-YP435351BPB
CSB-EP435351BPB
CSB-BP435351BPB
CSB-MP435351BPB
CSB-EP435351BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Malate synthase G (glcB), partial CSB-YP435352BPB
CSB-EP435352BPB
CSB-BP435352BPB
CSB-MP435352BPB
CSB-EP435352BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii 50S ribosomal protein L15 (rplO) CSB-YP435353BPB
CSB-EP435353BPB
CSB-BP435353BPB
CSB-MP435353BPB
CSB-EP435353BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii 50S ribosomal protein L14 (rplN) CSB-YP435354BPB
CSB-EP435354BPB
CSB-BP435354BPB
CSB-MP435354BPB
CSB-EP435354BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Chromosome partition protein Smc (smc), partial CSB-YP435355BPB
CSB-EP435355BPB
CSB-BP435355BPB
CSB-MP435355BPB
CSB-EP435355BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Elongation factor 4 (lepA), partial CSB-YP435356BPB
CSB-EP435356BPB
CSB-BP435356BPB
CSB-MP435356BPB
CSB-EP435356BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Cell division protein FtsB (ftsB), partial CSB-YP435357BPB
CSB-EP435357BPB
CSB-BP435357BPB
CSB-MP435357BPB
CSB-EP435357BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii 50S ribosomal protein L23 (rplW) CSB-YP435358BPB
CSB-EP435358BPB
CSB-BP435358BPB
CSB-MP435358BPB
CSB-EP435358BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii 50S ribosomal protein L10 (rplJ) CSB-YP435359BPB
CSB-EP435359BPB
CSB-BP435359BPB
CSB-MP435359BPB
CSB-EP435359BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii 50S ribosomal protein L34 (rpmH) CSB-YP435360BPB
CSB-EP435360BPB
CSB-BP435360BPB
CSB-MP435360BPB
CSB-EP435360BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii ADP-L-glycero-D-manno-heptose-6-epimerase (hldD) CSB-YP435361BPB
CSB-EP435361BPB
CSB-BP435361BPB
CSB-MP435361BPB
CSB-EP435361BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum Lipoprotein signal peptidase (lspA), partial CSB-YP435362BOS1
CSB-EP435362BOS1
CSB-BP435362BOS1
CSB-MP435362BOS1
CSB-EP435362BOS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella tribocorum Heat-inducible transcription repressor HrcA (hrcA) CSB-YP435363BOS
CSB-EP435363BOS
CSB-BP435363BOS
CSB-MP435363BOS
CSB-EP435363BOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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